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Purification and mo...
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Christakopoulos, Paul
(författare)
Purification and mode of action of an alkali-resistant endo-1,4-β-glucanase from Bacillus pumilus
- Artikel/kapitelEngelska1999
Förlag, utgivningsår, omfång ...
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Elsevier BV,1999
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:ltu-10417
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https://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-10417URI
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https://doi.org/10.1006/abbi.1999.1102DOI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Upprättat; 1999; 20130218 (ysko)
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Alkaline endo-1,4-β-d-glucanase was secreted byBacillus pumilusgrown in submerged culture on a combination of oat spelt xylan and corn starch as carbon sources. The enzyme was purified to homogeneity by Sephacryl S-200 and Q-Sepharose column chromatography. The protein corresponded to molecular mass and pIvalues of 67 kDa and 3.7, respectively. The enzyme was optimally active at pH 7.0–8.0 and 60°C and retained 50% of its optimum activity at pH 12. The most notable characteristic of the endoglucanase was its high stability up to pH 12 for 20 h at 30°C. The enzyme hydrolyzed carboxymethylcellulose (CMC) and cello-oligosaccharides but was inactive on cellobiose, cellotriose, Avicel, xylan, 4-nitrophenyl-β-d-glucoside, 4-nitrophenyl-β-d-cellobioside, and 4-nitrophenyl-β-d-xyloside. Analysis of reaction mixtures by HPLC revealed that the enzyme produced almost exclusively cellotriose when acted on CMC and appeared to hydrolyze cello-oligosaccharides by successively releasing cellotriose. The use of 4-methylumbelliferyl cello-oligosaccharides and the determination of bond cleavage frequency revealed that the enzyme preferentially hydrolyzed the third glycosidic bond adjacent to the glycon. The enzyme mediated a decrease in the viscosity of CMC associated with a release of only small amounts of reducing sugar. The enzyme activity was not inhibited by metal ions, surfactants, and chelating agents used as components of laundry detergents.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Hatzinikolaou, Dimitris G.National Technical University of Athens
(författare)
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Fountoukidis, GeorgeNational Technical University of Athens
(författare)
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Kekos, DimitrisNational Technical University of Athens
(författare)
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Claeyssens, MarcUniversity of Ghent
(författare)
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Macris, Basil J.National Technical University of Athens
(författare)
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National Technical University of AthensUniversity of Ghent
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Archives of Biochemistry and Biophysics: Elsevier BV364:1, s. 61-660003-98611096-0384
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