Effects of snap-freezing and near-infrared laser illumination on porcine prostate tissue as measured by Raman spectroscopy

Validerad; 2009; 20081205 (ramser)
Most Raman spectroscopic studies on tissue are performed in vitro. To assure that the results are applicable to in vivo examinations, preparation protocols and measurement procedures of tissue for in vitro studies should preserve tissue characteristics close to the native state. This study had two aims. The first was to elucidate if photoinduced effects arise during 5 minutes' continuous illumination of tissue with an 830 nm laser at an irradiance of 3 × 1010 W/m2. The second was to investigate the effects of snap-freezing of porcine prostate tissue in liquid nitrogen and subsequent storage at -80 °C, by means of multivariate analysis. 830 nm laser illumination of the specified irradiance did not affect the Raman spectra. A decrease of the spectral background was observed, likely due to photobleaching of tissue fluorophores. Snap-freezing and subsequent storage at -80 °C gave rise to subtle but significant alterations in Raman spectra, most likely related to changes in the protein conformations

TEKNIK OCH TEKNOLOGIER Medicinteknik Annan medicinteknik hsv//swe
ENGINEERING AND TECHNOLOGY Medical Engineering Other Medical Engineering hsv//eng
Medical Engineering for Healthcare
Medicinsk teknik för hälsovård
Centre - Centre for Biomedical Engineering and Physics (CMTF)
Centrumbildning - Centrum för medicinsk teknik och fysik (CMTF)

Ramser, KerstinLuleå tekniska universitet,Signaler och system(Swepub:ltu)ramser (författare)
Lindahl, Olof (författare)
Luleå tekniska universitetSignaler och system (creator_code:org_t)

Ingår i:The Analyst: Royal Society of Chemistry (RSC)134:9, s. 1815-18210003-26541364-5528

Av författaren/redakt...: Candefjord, Stef ...; Ramser, Kerstin; Lindahl, Olof

Om ämnet

TEKNIK OCH TEKNOLOGIER: TEKNIK OCH TEKNO ...; och Medicinteknik; och Annan medicintek ...

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