id:"swepub:oai:DiVA.org:ltu-11465"
Search: id:"swepub:oai:DiVA.org:ltu-11465" > Production of the b...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Enman, JosefineLuleå tekniska universitet,Kemiteknik (author)
Production of the bioactive compound eritadenine by submerged cultivation of shiitake (Lentinus edodes) mycelia
- Article/chapterEnglish2008
Publisher, publication year, extent ...
- 2008-03-26
- American Chemical Society (ACS),2008
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:ltu-11465
- https://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-11465URI
- https://doi.org/10.1021/jf800091aDOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Validerad; 2008; 20080429 (joen)
- Fruit bodies and mycelia of shiitake mushroom (Lentinus edodes) have been shown to contain the cholesterol-reducing compound eritadenine, 2(R),3(R)-dihydroxy-4-(9-adenyl)butyric acid. In the search for a production method for eritadenine, shiitake mycelia were investigated in the present study. The mycelia were cultivated both in shake flasks and in bioreactors, to investigate the effects of pH, stirring rate, and reactor type on the production and distribution of eritadenine. Both the biomass and the culture broth were examined for their eritadenine content. In the shake flasks, the final concentration of eritadenine was 1.76 mg/L and eritadenine was equally distributed between the mycelia and the growth media. In the bioreactors, the shiitake mycelia were found to contain eritadenine in relatively low levels, whereas the majority, 90.6-98.9%, was detected in the growth media. Applying a stirring rate of 250 rpm during bioreactor cultivation resulted in the highest eritadenine concentrations: 10.23 mg/L when the pH was uncontrolled and 9.59 mg/L when the pH was controlled at 5.7. Reducing the stirring rate to 50 rpm resulted in a decreased eritadenine concentration, both at pH 5.7 (5.25 mg/L) and when pH was not controlled (5.50 mg/L). The mycelia in the shake flask cultures appeared as macroscopic aggregates, whereas mycelia cultivated in bioreactors grew more as freely dispersed filaments. This study demonstrates for the first time the extra- and intracellular distribution of eritadenine produced by shiitake mycelial culture and the influence of reactor conditions on the mycelial morphology and eritadenine concentrations.
Subject headings and genre
- TEKNIK OCH TEKNOLOGIER Industriell bioteknik Bioprocessteknik hsv//swe
- ENGINEERING AND TECHNOLOGY Industrial Biotechnology Bioprocess Technology hsv//eng
- Biokemisk processteknik
- Biochemical Process Engineering
Added entries (persons, corporate bodies, meetings, titles ...)
- Hodge, David (author)
- Berglund, KrisLuleå tekniska universitet,Industriell miljö- och processteknik(Swepub:ltu)krib (author)
- Rova, Ulrika (author)
- Luleå tekniska universitetKemiteknik (creator_code:org_t)
Related titles
- In:Journal of Agricultural and Food Chemistry: American Chemical Society (ACS)56:8, s. 2609-26120021-85611520-5118
Internet link
Find in a library
- Journal of Agricultural and Food Chemistry (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Enman, Josefine
- Hodge, David
- Berglund, Kris
- Rova, Ulrika
- About the subject
-
- ENGINEERING AND TECHNOLOGY
- ENGINEERING AND ...
- and Industrial Biote ...
- and Bioprocess Techn ...
- Articles in the publication
- Journal of Agric ...
- By the university
- Luleå University of Technology
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
