Fractionation of whey-derived peptides using a combination of ultrafiltration and nanofiltration

Butylina, SvetlanaDepartment of Chemical Technology, Lappeenranta University of Technology (author)

This paper describes the fractionation and further isolation and characterisation of peptides and proteins present in sweet whey by means of ultrafiltration using a regenerated cellulose membrane with a nominal molar mass cut-off value of 10 kg/mol and nanofiltration through sulphonated polyether sulphone membrane with a cut-off of 1 kg/mol. The concentration of whey proteins was done below the critical flux. The sieving coefficients for the whey components (proteins, lactose and salts) were estimated. Whey proteins were completely rejected by the ultrafiltration membrane. Size exclusion chromatography (SEC) and matrix-assisted laser desorption ionisation time-of-flight (MALDI-TOF) mass spectrometry were used to evaluate the molar masses of the peptide fractions that were present in the whey permeates. Nanofiltration of whey permeates obtained after ultrafiltration was conducted at two pH values (9.5 and 3.0) that corresponded to the different charged states of the membrane and of the peptides. The transmission of peptides, amino acids and lactose was found to be mainly affected by the permeability of the fouling layer. The selectivity of the nanofiltration membranes toward peptides compared to lactose was calculated as 0.82 and 6.81 at pH 9.5 and 3.0, respectively.

Luque, SusanabDepartment of Chemical and Environmental Engineering, University of Oviedo (author)
Nyström, MarianneDepartment of Chemical Technology, Lappeenranta University of Technology (author)
Department of Chemical Technology, Lappeenranta University of TechnologybDepartment of Chemical and Environmental Engineering, University of Oviedo (creator_code:org_t)

By the author/editor: Butylina, Svetla ...; Luque, Susana; Nyström, Mariann ...

About the subject

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Bio Materials

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