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Recovery of l-Argin...
Recovery of l-Arginine from Model Solutions and Fermentation Broth Using Zeolite-Y Adsorbent
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- Faisal, Abrar (författare)
- Luleå tekniska universitet,Kemiteknik
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- Holmlund, Mattias (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,Swedish University of Agriculture Sciences
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- Ginesy, Mireille (författare)
- Luleå tekniska universitet,Kemiteknik
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- Holmgren, Allan (författare)
- Luleå tekniska universitet,Kemiteknik
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- Enman, Josefine (författare)
- Luleå tekniska universitet,Kemiteknik
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- Hedlund, Jonas (författare)
- Luleå tekniska universitet,Kemiteknik
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- Grahn, Mattias (författare)
- Luleå tekniska universitet,Kemiteknik
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(creator_code:org_t)
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- 2019-04-15
- 2019
- Engelska.
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Ingår i: ACS Sustainable Chemistry and Engineering. - : American Chemical Society (ACS). - 2168-0485. ; 7:9, s. 8900-8907
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Ämnesord
Stäng
- Arginine was produced via fermentation of sugars using the engineered microorganism Escherichia coli. Zeolite-Y adsorbents in the form of powder and extrudates were used to recover arginine from both a real fermentation broth and aqueous model solutions. An adsorption isotherm was determined using model solutions and zeolite-Y powder. The saturation loading was determined to be 0.2 g/g using the Sips model. Arginine adsorbed from a real fermentation broth using either zeolite-Y powder or extrudates both showed a maximum loading of 0.15 g/g at pH 11. This adsorbed loading is very close to the corresponding value obtained from the model solution showing that under the experimental conditions the presence of additional components in the broth did not have a significant effect on the adsorption of arginine. Furthermore, a breakthrough curve was determined for extrudates using a 1 wt % arginine model solution. The selectivity for arginine over ammonia and alanine from the real fermentation broth at pH 11 was 1.9 and 8.3, respectively, for powder, and 1.0, and 4.1, respectively, for extrudates. To the best of our knowledge, this is the first time recovery of arginine from real fermentation broths using any type of adsorbent has been reported.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Kemiska processer (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Chemical Process Engineering (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioprocessteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioprocess Technology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Annan kemiteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Other Chemical Engineering (hsv//eng)
Nyckelord
- Arginine
- FAU
- Fermentation broth
- Escherichia coli
- Adsorption
- Selectivity
- Breakthrough
- Chemical Technology
- Kemisk teknologi
- Biokemisk processteknik
- Biochemical Process Engineering
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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