The crystal structure of a Fusarium oxysporum feruloyl esterase that belongs to the tannase family

Dimarogona, MariaInstitute of Chemical Biology, National Hellenic Research Foundation, Athens, Greece. School of Chemical Engineering, National Technical University of Athens, Greece (författare)

Validerad;2020;Nivå 2;2020-06-15 (alebob)
Feruloyl esterases are enzymes of industrial interest that catalyse the hydrolysis of the ester bond between hydroxycinnamic acids such as ferulic acid and sugars present in the plant cell wall. Although there are several structures of biochemically characterized feruloyl esterases available, the structural determinants of their substrate specificity are not yet fully understood. Here, we present the crystal structure of a feruloyl esterase from Fusarium oxysporum (FoFaeC) at 2.3 Å resolution. Similar to the two other tannase‐like feruloyl esterases, FoFaeC features a large lid domain covering the active site with potential regulatory role and a disulphide bond that brings together the serine and histidine of the catalytic triad. Differences are mainly observed in the metal coordination site and the substrate binding pocket.

TEKNIK OCH TEKNOLOGIER Industriell bioteknik Bioprocessteknik hsv//swe
ENGINEERING AND TECHNOLOGY Industrial Biotechnology Bioprocess Technology hsv//eng
biocatalysis
feruloyl esterase
Fusarium oxysporum
tannase family
X‐ray crystallography
Biokemisk processteknik
Biochemical Process Engineering

Topakas, EvangelosSchool of Chemical Engineering, National Technical University of Athens, Greece (författare)
Christakopoulos, PaulLuleå tekniska universitet,Kemiteknik(Swepub:ltu)pauchr (författare)
Chrysina, Evangelia D.Institute of Chemical Biology, National Hellenic Research Foundation, Athens, Greece (författare)
Institute of Chemical Biology, National Hellenic Research Foundation, Athens, Greece. School of Chemical Engineering, National Technical University of Athens, GreeceSchool of Chemical Engineering, National Technical University of Athens, Greece (creator_code:org_t)

Av författaren/redakt...: Dimarogona, Mari ...; Topakas, Evangel ...; Christakopoulos, ...; Chrysina, Evange ...

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TEKNIK OCH TEKNOLOGIER: TEKNIK OCH TEKNO ...; och Industriell biot ...; och Bioprocessteknik

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