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Quantification of t...
Quantification of the Monomer Compositions of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Poly(3-hydroxyvalerate) by Alkaline Hydrolysis and Using High-Performance Liquid Chromatography
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- Saito, Kyo (författare)
- Course of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan
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- Reddy, M. Venkateswar (författare)
- Department of Civil and Environmental Engineering, Colorado State University, Fort Collins, CO 80523, USA
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- Sarkar, Omprakash (författare)
- Luleå tekniska universitet,Kemiteknik
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- Kumar, A. Naresh (författare)
- Department of Environmental Science and Technology, University of Maryland, College Park, MD 20742, USA
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- Choi, DuBok (författare)
- Faculty of Advanced Industry Convergence, Chosun University, Kwangju 61452, Republic of Korea
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- Chang, Young-Cheol (författare)
- Course of Chemical and Biological Engineering, Division of Sustainable and Environmental Engineering, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan
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(creator_code:org_t)
- Mdpi, 2023
- 2023
- Engelska.
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Ingår i: Bioengineering. - : Mdpi. - 2306-5354. ; 10:5
- Relaterad länk:
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https://ltu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- With the growing interest in bioplastics, there is an urgent need to develop rapid analysis methods linked to production technology development. This study focused on the production of a commercially non-available homopolymer, poly(3-hydroxyvalerate) (P(3HV)), and a commercially available copolymer, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(3HB-co-3HV)), through fermentation using two different bacterial strains. The bacteria Chromobacterium violaceum and Bacillus sp. CYR1 were used to produce P(3HV) and P(3HB-co-3HV), respectively. The bacterium Bacillus sp. CYR1 produced 415 mg/L of P(3HB-co-3HV) when incubated with acetic acid and valeric acid as the carbon sources, whereas the bacterium C. violaceum produced 0.198 g of P(3HV)/g dry biomass when incubated with sodium valerate as the carbon source. Additionally, we developed a fast, simple, and inexpensive method to quantify P(3HV) and P(3HB-co-3HV) using high-performance liquid chromatography (HPLC). As the alkaline decomposition of P(3HB-co-3HV) releases 2-butenoic acid (2BE) and 2-pentenoic acid (2PE), we were able to determine the concentration using HPLC. Moreover, calibration curves were prepared using standard 2BE and 2PE, along with sample 2BE and 2PE produced by the alkaline decomposition of poly(3-hydroxybutyrate) and P(3HV), respectively. Finally, the HPLC results obtained by our new method were compared using gas chromatography (GC) analysis.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Kemiteknik -- Polymerteknologi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Chemical Engineering -- Polymer Technologies (hsv//eng)
Nyckelord
- chromatography
- crotonic acid
- homopolymer
- copolymer
- polyhydroxyalkanoate
- poly(3-hydroxyvalerate)
- Biokemisk processteknik
- Biochemical Process Engineering
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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