Human lipoproteins at model cell membranes: Role of the lipoprotein class on lipid dynamics

Browning, T. K.Department of Pharmacy, Uppsala University, Uppsala, Sweden (författare)

Human lipoproteins at model cell membranes : Role of the lipoprotein class on lipid dynamics

High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the ‘good’ cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the ‘bad’ cholesterol, depositing lipid material into the vascular wall.

Lind, Tania KjellerupMalmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)ag5616 (författare)
Maric, SelmaMalmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)af4045 (författare)
Malekkhaiat-Häffner, S.Department of Pharmacy, Uppsala University, Uppsala, Sweden (författare)
Fredrikson, G. N.Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden (författare)
Bengtsson, E.Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden (författare)
Malmsten, M.Department of Pharmacy, Uppsala University, Uppsala, Sweden; Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark (författare)
Cárdenas, MaritéMalmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)ae0614 (författare)
Department of Pharmacy, Uppsala University, Uppsala, SwedenInstitutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)

Av författaren/redakt...: Browning, T. K.; Lind, Tania Kjel ...; Maric, Selma; Malekkhaiat-Häff ...; Fredrikson, G. N ...; Bengtsson, E.; visa fler...; Malmsten, M.; Cárdenas, Marité; visa färre...

Om ämnet

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.