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LPS induces GROα ch...
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Jönsson, DanielMalmö högskola,Odontologiska fakulteten (OD)
(author)
LPS induces GROα chemokine production via NFκB in oral fibroblasts
- Article/chapterEnglish2009
Publisher, publication year, extent ...
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2009-05-09
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Springer,2009
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:mau-15426
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https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-15426URI
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https://doi.org/10.1007/s00011-009-0049-zDOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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OBJECTIVE AND DESIGN: Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GROalpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GROalpha by oral fibroblasts have not previously been presented. MATERIALS AND METHODS: GROalpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. RESULTS: We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GROalpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GROalpha and GROalpha protein. The steroid hormone estrogen had no effect on LPS-induced GROalpha mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GROalpha production, and the NF-kappaB blocker MG 132 fully prevented LPS-induced GROalpha. CONCLUSIONS: Oral fibroblasts respond to LPS stimulation by increasing GROalpha production via the transcription factor NF-kappaB, suggesting that this mechanism may be involved in development of periodontal inflammation.
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Amisten, Stefan
(author)
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Bratthall, Gunilla
(author)
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Holm, Anders
(author)
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Nilsson, Bengt-Olof
(author)
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Malmö högskolaOdontologiska fakulteten (OD)
(creator_code:org_t)
Related titles
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In:Inflammation Research: Springer11:58, s. 791-7961023-38301420-908X
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