Filter Plate–Based Screening of MIP SPE Materials for Capture of the Biomarker Pro-Gastrin-Releasing Peptide

Jagadeesan, KishoreLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH (författare)

Affinity-based solid-phase extraction (SPE) is an attractive low-cost sample preparation strategy for biomarker analysis. Molecularly imprinted polymers (MIPs) as affinity sorbents offer unique opportunities for affinity SPE, due to their low manufacturing cost and high robustness. A limitation is the prediction of their affinity; therefore, screening of analyte recovery and specificity within a large range of SPE conditions is important in order to ensure high-sensitivity detection and assay reproducibility. Here, a µ-SPE method for screening of the MIP-SPE materials using a commercial 384-well filter plate is presented. The method allows for rapid and automated screening using 10?30 µL of packed SPE sorbent per well and sample volumes in the range of 10?70 µL. This enables screening of many different SPE sorbents while simultaneously identifying optimal SPE conditions. In addition, the 384-well format also facilitates detection with a multitude of analytical platforms. Performance of the µ-MIP-SP

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
TEKNIK OCH TEKNOLOGIER Medicinteknik Medicinsk laboratorie- och mätteknik hsv//swe
ENGINEERING AND TECHNOLOGY Medical Engineering Medical Laboratory and Measurements Technologies hsv//eng
sample preparation
mass spectrometry
separations or chromatography
protein chemistry
protein labeling
proteomics
biomarkers
Sample preparation
mass spectromery
SEPARATIONS
chromatography
protein chemistry
Biomarkers
protein labeling

Rossetti, CeciliaDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway (författare)
Qader, Abed AbdelDepartment of Environmental Chemistry and Analytical Chemistry, Institute for Environmental Research (INFU), Technical University of Dortmund, Dortmund, Germany (författare)
Reubsaet, LeonDepartment of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, Oslo, Norway (författare)
Sellergren, BörjeMalmö University,Malmö högskola,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)ab8456 (författare)
Laurell, ThomasLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-tla (författare)
Ekström, SimonLund University,Lunds universitet,Avdelningen för Biomedicinsk teknik,Institutionen för biomedicinsk teknik,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Biomedical Engineering,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-sek (författare)
Avdelningen för Biomedicinsk teknikInstitutionen för biomedicinsk teknik (creator_code:org_t)

Av författaren/redakt...: Jagadeesan, Kish ...; Rossetti, Cecili ...; Qader, Abed Abde ...; Reubsaet, Leon; Sellergren, Börj ...; Laurell, Thomas; visa fler...; Ekström, Simon; visa färre...

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TEKNIK OCH TEKNOLOGIER: TEKNIK OCH TEKNO ...; och Medicinteknik; och Medicinsk labora ...

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