The Protein Corona Leads to Deformation of Spherical Micelles

Cao, ChengCentre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney, 2052, Australia (författare)

The formation of a non-specific protein corona around nanoparticles (NPs) has been identified as one of the culprits for failed nanomedicine. The amount and type of adsorbed protein from the blood plasma are known to determine the fate of NPs and the accessibility of targeting ligands. Herein, we show that the adsorbed protein may not only enlarge the NPs and change their surface properties but also, in the case of soft NPs such as polymer micelles, lead to deformation. Poly(1-O-methacryloyl -beta-D-fructopyranose)-b-poly(methylmethacrylate) (P(1-O-MAFru)-b-PMMA) block co-polymers were self-assembled into NPs with a spherical core-shell morphology as determined by small angle neutron scattering (SANS). Upon incubation with albumin, TEM, SANS, and small angle X-ray scattering (SAXS) revealed the adsorption of albumin and deformation of the NPs with a spheroid geometry. Removal of the protein led to the reversal of the morphology back to the spherical core-shell structure. Structural studies and cell studies of uptake of the NPs imply that the observed deformation may influence blood circulation time and cell uptake.

Zhang, LinCentre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney, 2052, Australia (författare)
Kent, BenCentre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney, 2052, Australia (författare)
Wong, SandyCentre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney, 2052, Australia (författare)
Garvey, Christopher J.Malmö University,Lund University,Lunds universitet,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Australian Centre for Neutron Scattering, Australia Nuclear Science and Technology Organisation, Lucas Heights, 2234, Australia; Lund Institute for Advanced Neutron and X-ray Science, Lund, 22100, Sweden,LINXS - Institute of advanced Neutron and X-ray Science,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)ch8532ga (författare)
Stenzel, Martina H.Centre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney, 2052, Australia (författare)
Centre for Advanced Macromolecular Design, School of Chemistry, The University of New South Wales, Sydney, 2052, AustraliaInstitutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)

Ingår i:Angewandte Chemie International Edition: John Wiley & Sons60:18, s. 10342-103491433-78511521-3773

Av författaren/redakt...: Cao, Cheng; Zhang, Lin; Kent, Ben; Wong, Sandy; Garvey, Christop ...; Stenzel, Martina ...

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