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Temperature and salt controlled tuning of protein clusters

Beck, Christian (author)
Univ Tubingen, Inst Angew Phys, Morgenstelle 10, D-72076 Tubingen, Germany.;Inst Max von Laue Paul Langevin, 71 Ave Martyrs, F-38042 Grenoble, France.,Institut Laue Langevin,University of Tübingen
Grimaldo, Marco (author)
Inst Max von Laue Paul Langevin, 71 Ave Martyrs, F-38042 Grenoble, France.,Institut Laue Langevin
Braun, Michal K. (author)
Univ Tubingen, Inst Angew Phys, Morgenstelle 10, D-72076 Tubingen, Germany.,University of Tübingen
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Buhl, Lena (author)
Univ Tubingen, Inst Angew Phys, Morgenstelle 10, D-72076 Tubingen, Germany.,University of Tübingen
Matsarskaia, Olga (author)
Inst Max von Laue Paul Langevin, 71 Ave Martyrs, F-38042 Grenoble, France.,Institut Laue Langevin
Jalarvo, Niina H. (author)
Forschungszentrum Julich, Julich Ctr Neutron Sci JCNS, D-52425 Julich, Germany.;Oak Ridge Natl Lab, Chem & Engn Mat Div, Neutron Sci Directorate, POB 2008, Oak Ridge, TN 37831 USA.;Oak Ridge Natl Lab, JCNS Outstn Spallat Neutron Source SNS, POB 2008, Oak Ridge, TN 37831 USA.,Jülich Research Centre,Oak Ridge National Laboratory
Zhang, Fajun (author)
Univ Tubingen, Inst Angew Phys, Morgenstelle 10, D-72076 Tubingen, Germany.,University of Tübingen
Roosen-Runge, Felix (author)
Malmö University,Lund University,Lunds universitet,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Lund Univ, Div Phys Chem, Nat Vetarvagen 14, S-22100 Lund, Sweden.,Fysikalisk kemi,Enheten för fysikalisk och teoretisk kemi,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Physical Chemistry,Physical and theoretical chemistry,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
Schreiber, Frank (author)
Univ Tubingen, Inst Angew Phys, Morgenstelle 10, D-72076 Tubingen, Germany.,University of Tübingen
Seydel, Tilo (author)
Inst Max von Laue Paul Langevin, 71 Ave Martyrs, F-38042 Grenoble, France.,Institut Laue Langevin
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Univ Tubingen, Inst Angew Phys, Morgenstelle 10, D-72076 Tubingen, Germany;Inst Max von Laue Paul Langevin, 71 Ave Martyrs, F-38042 Grenoble, France. Institut Laue Langevin (creator_code:org_t)
2021
2021
English.
In: Soft Matter. - : Royal Society of Chemistry. - 1744-683X .- 1744-6848. ; :37, s. 8506-8516
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • The formation of molecular assemblies in protein solutions is of strong interest both from a fundamental viewpoint and for biomedical applications. While ordered and desired protein assemblies are indispensable for some biological functions, undesired protein condensation can induce serious diseases. As a common cofactor, the presence of salt ions is essential for some biological processes involving proteins, and in aqueous suspensions of proteins can also give rise to complex phase diagrams including homogeneous solutions, large aggregates, and dissolution regimes. Here, we systematically study the cluster formation approaching the phase separation in aqueous solutions of the globular protein BSA as a function of temperature (T), the protein concentration (c(p)) and the concentrations of the trivalent salts YCl3 and LaCl3 (c(s)). As an important complement to structural, i.e. time-averaged, techniques we employ a dynamical technique that can detect clusters even when they are transient on the order of a few nanoseconds. By employing incoherent neutron spectroscopy, we unambiguously determine the short-time self-diffusion of the protein clusters depending on c(p), c(s) and T. We determine the cluster size in terms of effective hydrodynamic radii as manifested by the cluster center-of-mass diffusion coefficients D. For both salts, we find a simple functional form D(c(p), c(s), T) in the parameter range explored. The calculated inter-particle attraction strength, determined from the microscopic and short-time diffusive properties of the samples, increases with salt concentration and temperature in the regime investigated and can be linked to the macroscopic behavior of the samples.

Subject headings

NATURVETENSKAP  -- Kemi -- Fysikalisk kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences -- Physical Chemistry (hsv//eng)

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