Glycosylation in cancer and infection: the role of sialic acid

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: id:"swepub:oai:DiVA.org:mau-46316" > Glycosylation in ca...

1 av 1
Föregående post
Nästa post
Till träfflistan

Zhang, YuechengMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV) (författare)

Glycosylation in cancer and infection : the role of sialic acid

BokEngelska2021

Förlag, utgivningsår, omfång ...

Malmo :Malmö universitet,2021
58 s.
electronicrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:mau-46316
ISBN:9789178772209
ISBN:9789178772216
https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-46316URI
https://doi.org/10.24834/isbn.9789178772216DOI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:vet swepub-contenttype
Ämneskategori:dok swepub-publicationtype

Serie

Malmö University Health and Society Dissertations,1653-5383 ;2021:7

Anmärkningar

Sialic acids (SA), a group of nine-carbon backbone monosaccharides are abundantly expressed in vertebrates. They are usually linked to the terminal of glycan chains and play crucial roles in many biological processes, including cell adhesion, cell-cell interactions, immune modulation, cancer cell migration and invasion, as well as viral infections. To analyze and monitor SA expression, antibodies and glycan-binding lectins are typically used. However, high costs and poor stability limit the application in SA analysis. To overcome these drawbacks, an imprinting technique was used to synthesize an alternative SA receptor – SA molecularly imprinted polymers (SA-MIPs). Fluorescent molecules are embedded into the MIPs, facilitating the detection of MIPs binding to cells by flow cytometry and fluorescence microscopy. Firstly, core-shell SA imprinted MIPs were used to analyze SA expression in a panel of breast cancer cell lines. The SA expression of these cell lines was also tested by using the two glycan-binding lectins, MAL andSNA, which recognize α2,3 and α2,6 SA, respectively. Our results show that breast cancer cell lines express α2,3 and α2,6 SA dissimilarly, and hence present different SA-MIP binding patterns. The specificity of SA-MIPs was further verified by an inhibition assay using two pentavalent SA conjugates that interfere with the SAMIPs.Furthermore, the SA-MIP synthesis protocol has been improved by using silica-coated polystyrene particles. The polystyrene core particles are lighter and smaller, increasing MIP suspensibility and augmenting MIP-cancer cell interactions. The cancer cell binding properties and the specificity have been verified by using thirteen different cancer cell lines, showing that the SA-MIPs can be used as effective tools for SA expression analysis. The SA-MIPs were used to analyze the SA expression of in vitro cultured cells treated with soluble cytokines to mimic the tumor microenvironment. The SA expression of two cancer cell lines stimulated with soluble cytokines was analyzed by using lectins and SA-MIPs. The MIPbinding data correlated well with lectin staining results, demonstrating the potential of SA-MIPs to be used in the analysis of overexpressed SA in the tumor microenvironment. Furthermore, the involvement of SA in the infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was assessed. The viral surface receptor-binding domain (RBD) recognizes and conjugates with receptors on host cells, triggering the infection. Although the interaction between the RBD and host cells has been extensively studied, the mechanism behind this reaction is not fully determined. In this study, the interaction between the viral RBD and a panel of human cell lines from tissues susceptible to viral infection was tested. Moreover, the role of SA in this interaction has also been tested and evaluated.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Biomaterialvetenskap hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Biomaterials Science hsv//eng
NATURVETENSKAP Biologi Cellbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Cell Biology hsv//eng
Cancer
sialic acids
receptor
molecularly imprinted polymers
SARS-CoV-2
fluorescence
Health and society
Hälsa och samhälle

Biuppslag (personer, institutioner, konferenser, titlar ...)

Wingren, Anette,ProfessorMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV) (preses)
Stollenwerk, Maria,Senior lecturerMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV) (preses)
Ohlsson, Lars,Associate ProfessorMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV) (preses)
Dufva, Martin,Associate ProfessorDepartment of Health Technology, Technical University of Denmark (opponent)
Malmö universitetInstitutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)

Internetlänk

Hitta via bibliotek

Glycosylation in cancer and infection the role of sialic acid (Sök publikationen i LIBRIS)

Till lärosätets databas

1 av 1
Föregående post
Nästa post
Till träfflistan

Hitta mer i SwePub

Av författaren/redakt...: Zhang, Yuecheng; Wingren, Anette, ...; Stollenwerk, Mar ...; Ohlsson, Lars, A ...; Dufva, Martin, A ...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinsk biotek ...; och Biomaterialveten ...

NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Cellbiologi

Delar i serien: Malmö University ...

Av lärosätet: Malmö universitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se