Tracing Aluminium-based Adjuvants: Their Interactions with Immune Competent Cells and their Effect on Mitochondrial Activity

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Sökning: id:"swepub:oai:DiVA.org:mau-5015" > Tracing Aluminium-b...

1 av 1
Föregående post
Nästa post
Till träfflistan

Danielsson, RaviMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV) (författare)

Tracing Aluminium-based Adjuvants : Their Interactions with Immune Competent Cells and their Effect on Mitochondrial Activity

Artikel/kapitelEngelska2018

Förlag, utgivningsår, omfång ...

Bentham eBooks,2018
electronicrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:mau-5015
https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-5015URI
https://doi.org/10.2174/1874226201808010001DOI

Kompletterande språkuppgifter

Språk:engelska
Sammanfattning på:engelska

Ingår i deldatabas

SwePubSwePub

Klassifikation

Ämneskategori:ref swepub-contenttype
Ämneskategori:art swepub-publicationtype

Anmärkningar

Background: Studies revealing the immune stimulatory properties of aluminium-based adjuvants (ABAs) have been impaired by the absence of simple and reliable methods of tracing the adjuvants and their effect on biochemical processes upon endocytosis. Objective: To verify that labelling of ABAs with lumogallion doesn’t affect the physicochemical properties of the adjuvant; tracing cellular interaction with aluminium adjuvants; explore their effect on metabolic activity upon endocytosis. Methods: Physicochemical characterization by Z-potential and size distribution of ABAs labelled with lumogallion. Cellular interactions with ABAs by flow cytometry and confocal microscopy. Metabolic activity explored by measuring transformation of tetrazolium into formazan. Results: No or minor change of zeta potential and average particle size of lumogallion labelled aluminium oxyhydroxide, AlO(OH) and aluminium hydroxyphosphate, Al(OH)x(PO4 )y. Both phagocytosing and non-phagocytosing leukocytes became associated with ABAs at concentrations expected after in vivo administration of a vaccine. The ABAs were relatively toxic, affecting both lymphocytes and monocytes, and Al(OH)x(PO4 )y was more toxic than AlO(OH). Endocytosed aluminium adjuvant particles were not secreted from the cells and remained intracellular throughout several cell divisions. The presence of ABAs increased the mitochondrial activity of the monocytic cell line THP-1 and peripheral monocytes, as based on the transformation of tetrazolium into formazan. Conclusion: Lumogallion labelled ABAs is a valuable tool tracing interactions between ABAs and cells. Labelled ABAs can be traced intracellularly and ABAs are likely to remain intracellular for a long period of time. Intracellular ABAs increase the mitochondrial activity and the presence of intracellular Al ions is suggested to cause an increased mitochondrial activity. Keywords: Aluminium based adjuvant, Lumogallion, Mitochondrial activity, MTT assay, Phagocytosis, ABAS, Zeolites.

Ämnesord och genrebeteckningar

Aluminium based adjuvant
Lumogallion
Mitochondrial activity
MTT assay
Phagocytosis
ABAS
Zeolites

Biuppslag (personer, institutioner, konferenser, titlar ...)

Svensson, Andreas (författare)
Falkman, PeterMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV)(Swepub:mau)hsperni (författare)
Eriksson, HåkanMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV)(Swepub:mau)hshaer (författare)
Malmö universitetInstitutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)

Sammanhörande titlar

Ingår i:Open Immunology Journal: Bentham eBooks8, s. 1-151874-2262

Internetlänk

Hitta via bibliotek

Open Immunology Journal (Sök värdpublikationen i LIBRIS)

Till lärosätets databas

1 av 1
Föregående post
Nästa post
Till träfflistan

Hitta mer i SwePub

Av författaren/redakt...: Danielsson, Ravi; Svensson, Andrea ...; Falkman, Peter; Eriksson, Håkan

Artiklar i publikationen: Open Immunology ...

Av lärosätet: Malmö universitet

Sök utanför SwePub

Sök vidare i:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se