Enhanced selective capture of phosphomonoester lipids enabling highly sensitive detection of sphingosine 1-phosphate

Grasso, GiulianaMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Department of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, SA, Italy (författare)

Sphingolipids play crucial roles in cellular membranes, myelin stability, and signalling responses to physiological cues and stress. Among them, sphingosine 1-phosphate (S1P) has been recognized as a relevant biomarker for neurodegenerative diseases, and its analogue FTY-720 has been approved by the FDA for the treatment of relapsing-remitting multiple sclerosis. Focusing on these targets, we here report three novel polymeric capture phases for the selective extraction of the natural biomarker and its analogue drug. To enhance analytical performance, we employed different synthetic approaches using a cationic monomer and a hydrophobic copolymer of styrene-DVB. Results have demonstrated high affinity of the sorbents towards S1P and fingolimod phosphate (FTY-720-P, FP). This evidence proved that lipids containing phosphate diester moiety in their structures did not constitute obstacles for the interaction of phosphate monoester lipids when loaded into an SPE cartridge. Our suggested approach offers a valuable tool for developing efficient analytical procedures.

Sommella, Eduardo MDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, SA, Italy (författare)
Merciai, FabrizioDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, SA, Italy (författare)
Abouhany, RahmaMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)aj3358 (författare)
Shinde, Sudhirkumar AMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,School of Consciousness, Dr. Vishwanath Karad MIT World Peace University, 411038, Pune, India(Swepub:mau)ad7127 (författare)
Campiglia, PietroDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, SA, Italy (författare)
Sellergren, BörjeMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces(Swepub:mau)ab8456 (författare)
Crescenzi, CarloDepartment of Pharmacy, University of Salerno, Via Giovanni Paolo II 132, 84084, Fisciano, SA, Italy (författare)
Malmö universitetInstitutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)

Ingår i:Analytical and Bioanalytical Chemistry: Springer415:26, s. 6573-65821618-26421618-2650

Av författaren/redakt...: Grasso, Giuliana; Sommella, Eduard ...; Merciai, Fabrizi ...; Abouhany, Rahma; Shinde, Sudhirku ...; Campiglia, Pietr ...; visa fler...; Sellergren, Börj ...; Crescenzi, Carlo; visa färre...

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