SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Tillerkvist Kristina)
 

Sökning: WFRF:(Tillerkvist Kristina) > (2023) > Tolerogenic dendrit...

  • Dao Nyesiga, GillianMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Idogen AB, Lund, Sweden,Idogen AB, Sweden; Malmo Univ, Sweden (författare)

Tolerogenic dendritic cells generated in vitro using a novel protocol mimicking mucosal tolerance mechanisms represent a potential therapeutic cell platform for induction of immune tolerance.

  • Artikel/kapitelEngelska2023

Förlag, utgivningsår, omfång ...

  • Frontiers Media S.A.2023
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:mau-63661
  • https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-63661URI
  • https://doi.org/10.3389/fimmu.2023.1045183DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-199127URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding Agencies|The authors declare financial support was received for the research, authorship, and/or publication of this article. This was supported by Knowledge Foundation, Horizon 2020 (grant no. 778 246), and Ingrid Asp Foundation. Funding for open access publicatio; Knowledge Foundation [778 246]; Horizon 2020; Ingrid Asp Foundation
  • Dendritic cells (DCs) are mediators between innate and adaptive immunity and vital in initiating and modulating antigen-specific immune responses. The most important site for induction of tolerance is the gut mucosa, where TGF-β, retinoic acid, and aryl hydrocarbon receptors collaborate in DCs to induce a tolerogenic phenotype. To mimic this, a novel combination of compounds – the synthetic aryl hydrocarbon receptor (AhR) agonist IGN-512 together with TGF-β and retinoic acid – was developed to create a platform technology for induction of tolerogenic DCs intended for treatment of several conditions caused by unwanted immune activation. These in vitro-generated cells, designated ItolDCs, are phenotypically characterized by their low expression of co-stimulatory and activating molecules along with high expression of tolerance-associated markers such as ILT3, CD103, and LAP, and a weak pro-inflammatory cytokine profile. When co-cultured with T cells and/or B cells, ItolDC-cultures contain higher frequencies of CD25+Foxp3+ regulatory T cells (Tregs), CD49b+LAG3+ ‘type 1 regulatory (Tr1) T cells, and IL-10-producing B cells and are less T cell stimulatory compared to cultures with matured DCs. Factor VIII (FVIII) and tetanus toxoid (TT) were used as model antigens to study ItolDC antigen-loading. ItolDCs can take up FVIII, process, and present FVIII peptides on HLA-DR. By loading both ItolDCs and mDCs with TT, antigen-specific T cell proliferation was observed. Cryo-preserved ItolDCs showed a stable tolerogenic phenotype that was maintained after stimulation with LPS, CD40L, or a pro-inflammatory cocktail. Moreover, exposure to other immune cells did not negatively impact ItolDCs’ expression of tolerogenic markers. In summary, a novel protocol was developed supporting the generation of a stable population of human DCs in vitro that exhibited a tolerogenic phenotype with an ability to increase proportions of induced regulatory T and B cells in mixed cultures. This protocol has the potential to constitute the base of a tolDC platform for inducing antigen-specific tolerance in disorders caused by undesired antigen-specific immune cell activation.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Pool, LienekeIdogen AB, Lund, Sweden (författare)
  • Englezou, Pavlos CIdogen AB, Lund, Sweden (författare)
  • Hylander, TereseIdogen AB, Lund, Sweden (författare)
  • Ohlsson, LarsMalmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Malmo Univ, Sweden(Swepub:mau)hslabr (författare)
  • Appelgren, DanielLinköpings universitet,Avdelningen för diagnostik och specialistmedicin,Medicinska fakulteten(Swepub:liu)danso84 (författare)
  • Sundstedt, AnetteIdogen AB, Lund, Sweden (författare)
  • Tillerkvist, KristinaIdogen AB, Lund, Sweden (författare)
  • Romedahl, Hanne RIdogen AB, Lund, Sweden (författare)
  • Wigren, MariaIdogen AB, Lund, Sweden (författare)
  • Malmö universitetInstitutionen för biomedicinsk vetenskap (BMV) (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Frontiers in Immunology: Frontiers Media S.A.141664-3224

Internetlänk

Hitta via bibliotek

Till lärosätets databas

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy