Bacteria counting with impedance spectroscopy in a micro probe station

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Jönsson, M.Uppsala universitet,Elektronik,Nanoteknologi och funktionella material,Uppsala University, Sweden (författare)

A method to quantify the density of viable biological cells in suspensions is presented. The method is implemented by low-frequency impedance spectroscopy and based on the finding that immobilized ions are released to move freely in the surrounding suspension when viable Escherichia coli cells are killed by a heat shock. The presented results show that an amount of ions corresponding to ∼2 × 108 unit charges are released per viable bacterium killed. A micro probe station with coplanar Ti electrodes was electrically characterized and used as a measuring unit for the impedance spectroscopy recordings. This unit is compatible with common microfabrication techniques and should enable the presented method to be employed using a flow-cell device for viable bacteria counting in miniaturized on-line monitoring systems.

TEKNIK OCH TEKNOLOGIER Materialteknik Annan materialteknik hsv//swe
ENGINEERING AND TECHNOLOGY Materials Engineering Other Materials Engineering hsv//eng
Acoustic impedance
Cell immobilization
Electrodes
Ions
Spectroscopic analysis
Titanium
Bacteria counting
Coplanar Ti electrodes
Heat shock
Low-frequency impedance spectroscopy
Micro probe station
Bacteria
Functional materials
Engineering Science with specialization in Nanotechnology and Functional Materials

Welch, KenUppsala universitet,Nanoteknologi och funktionella material,Uppsala University, Sweden(Swepub:uu)kewel087 (författare)
Hamp, SvenMälardalens högskola (författare)
Strömme, MariaUppsala universitet,Nanoteknologi och funktionella material,Uppsala University, Sweden(Swepub:uu)mst26208 (författare)
Uppsala universitetElektronik (creator_code:org_t)

Ingår i:Journal of Physical Chemistry B: American Chemical Society (ACS)110:20, s. 10165-101691520-61061520-5207

Av författaren/redakt...: Jönsson, M.; Welch, Ken; Hamp, Sven; Strömme, Maria

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