On the importance of controlling film architecture in detecting prostate specific antigen

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Graça, Juliana SantosFederal University of São Carlos, Center for Sciences and Technology for Sustainability, Sorocaba, SP, Brazil (författare)

On the importance of controlling film architecture in detecting prostate specific antigen

Artikel/kapitelEngelska2018

Förlag, utgivningsår, omfång ...

Elsevier BV,2018
printrdacarrier

Nummerbeteckningar

LIBRIS-ID:oai:DiVA.org:miun-32286
https://urn.kb.se/resolve?urn=urn:nbn:se:miun:diva-32286URI
https://doi.org/10.1016/j.apsusc.2017.10.122DOI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:art swepub-publicationtype

Anmärkningar

Immunosensors made with nanostructured films are promising for detecting cancer biomarkers, even at early stages of the disease, but this requires control of film architecture to preserve the biological activity of immobilized antibodies. In this study, we used electrochemical impedance spectroscopy (EIS) to detect Prostate Specific Antigen (PSA) with immunosensors produced with layer-by-layer (LbL) films containing anti-PSA antibodies in two distinct film architectures. The antibodies were either adsorbed from solutions in which they were free, or from solutions where they were incorporated into liposomes of dipalmitoyl phosphatidyl glycerol (DPPG). Incorporation into DPPG liposomes was confirmed with surface plasmon resonance experiments, while the importance of electrostatic interactions on the electrical response was highlighted using the Finite Difference Time-Domain Method (FDTD). The sensitivity of both architectures was sufficient to detect the threshold value to diagnose prostate cancer (ca. 4 ng mL−1). In contrast to expectation, the sensor with the antibodies incorporated into DPPG liposomes had lower sensitivity, though the range of concentrations amenable to detection increased, according to the fitting of the EIS data using the Langmuir-Freundlich adsorption model. The performance of the two film architectures was compared qualitatively by plotting the data with a multidimensional projection technique, which constitutes a generic approach for optimizing immunosensors and other types of sensors.

Ämnesord och genrebeteckningar

TEKNIK OCH TEKNOLOGIER Materialteknik hsv//swe
ENGINEERING AND TECHNOLOGY Materials Engineering hsv//eng
Electrochemical impedance spectroscopy
Finite difference time-domain method
Immunosensor
Layer-by-layer
Liposome

Biuppslag (personer, institutioner, konferenser, titlar ...)

Miyazaki, Celina MassumiFederal University of São Carlos, Center for Sciences and Technology for Sustainability, Sorocaba, SP, Brazil (författare)
Shimizu, Flavio M.University of São Paulo, São Carlos, SP, Brazil (författare)
Volpati, DiogoMittuniversitetet,Avdelningen för naturvetenskap(Swepub:miun)diovol (författare)
Mejía-Salazar, J. R.University of São Paulo, São Carlos, SP, Brazil (författare)
Oliveira Jr, Osvaldo N.University of São Paulo, São Carlos, SP, Brazil (författare)
Ferreira, MarystelaFederal University of São Carlos, Center for Sciences and Technology for Sustainability, Sorocaba, SP, Brazil (författare)
Federal University of São Carlos, Center for Sciences and Technology for Sustainability, Sorocaba, SP, BrazilUniversity of São Paulo, São Carlos, SP, Brazil (creator_code:org_t)

Sammanhörande titlar

Ingår i:Applied Surface Science: Elsevier BV434, s. 1175-11820169-43321873-5584

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