Fisher: a program for the detection of H/ACA snoRNAs using MFE secondary structure prediction and comparative genomics - assessment and update

BackgroundThe H/ACA family of small nucleolar RNAs (snoRNAs) plays a central role in guiding the pseudouridylation of ribosomal RNA (rRNA). In an effort to systematically identify the complete set of rRNA-modifying H/ACA snoRNAs from the genome sequence of the budding yeast, Saccharomyces cerevisiae, we developed a program - Fisher - and previously presented several candidate snoRNAs based on our analysis [1]. FindingsIn this report, we provide a brief update of this work, which was aborted after the publication of experimentally-identified snoRNAs [2] identical to candidates we had identified bioinformatically using Fisher. Our motivation for revisiting this work is to report on the status of the candidate snoRNAs described in [1], and secondly, to report that a modified version of Fisher together with the available multiple yeast genome sequences was able to correctly identify several H/ACA snoRNAs for modification sites not identified by the snoGPS program [3]. While we are no longer developing Fisher, we briefly consider the merits of the Fisher algorithm relative to snoGPS, which may be of use for workers considering pursuing a similar search strategy for the identification of small RNAs. The modified source code for Fisher is made available as supplementary material. ConclusionOur results confirm the validity of using minimum free energy (MFE) secondary structure prediction to guide comparative genomic screening for RNA families with few sequence constraints.

Edvardsson, SverkerMittuniversitetet,Institutionen för naturvetenskap, teknik och matematik (-2012)(Swepub:miun)sveedv (author)
Tamas, I (author)
Moulton, Vincent (author)
Poole, AMStockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik (author)
Uppsala universitetCentrum för bioinformatik (creator_code:org_t)

In:BMC Research Notes: Springer Science and Business Media LLC1:49, s. 1-81756-0500

By the author/editor: Freyhult, Eva; Edvardsson, Sver ...; Tamas, I; Moulton, Vincent; Poole, AM

About the subject

By the university: Mid Sweden University; Stockholm University; Uppsala University

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