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Sökning: WFRF:(Bachmann Radu) > (2022) > Akkermansia mucinip...

  • Bachmann, RaduMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), UCLouvain, Université Catholique de Louvain, Brussels, Belgium; Colorectal Surgery Unit, Cliniques Universitaires Saint-Luc, Brussels, Belgium (författare)

Akkermansia muciniphila Reduces Peritonitis and Improves Intestinal Tissue Wound Healing after a Colonic Transmural Defect by a MyD88-Dependent Mechanism

  • Artikel/kapitelEngelska2022

Förlag, utgivningsår, omfång ...

  • 2022-08-27
  • MDPI,2022
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:oru-101170
  • https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-101170URI
  • https://doi.org/10.3390/cells11172666DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding agencies:Fonds de la Recherche Scientifique - FNRS FNRS T.0030.21 J.0027.22 WELBIO-CR-2022A-02 WELBIO-CR-2019C-02R 30770923 40007505  Netherlands Organization for Scientific Research (NWO) 024.002.002
  • Anastomotic leakage is a major complication following colorectal surgery leading to peritonitis, complications, and mortality. Akkermansia muciniphila has shown beneficial effects on the gut barrier function. Whether A. muciniphila reduces peritonitis and mortality during colonic leakage is unknown. Whether A. muciniphila can directly modulate the expression of genes in the colonic mucosa in humans has never been studied. We investigated the effects of a pretreatment (14 days) with live A. muciniphila prior to surgical colonic perforation on peritonitis, mortality, and wound healing. We used mice with an inducible intestinal-epithelial-cell-specific deletion of MyD88 (IEC-MyD88 KO) to investigate the role of the innate immune system in this context. In a proof-of-concept pilot study, healthy humans were exposed to A. muciniphila for 2 h and colonic biopsies taken before and after colonic instillation for transcriptomic analysis. Seven days after colonic perforation, A.-muciniphila-treated mice had significantly lower mortality and severity of peritonitis. This effect was associated with significant improvements of wound histological healing scores, higher production of IL22, but no changes in the mucus layer thickness or genes involved in cell renewal, proliferation, or differentiation. All these effects were abolished in IEC-MyD88 KO mice. Finally, human subjects exposed to A. muciniphila exhibited an increased level of the bacterium at the mucus level 2 h after instillation and significant changes in the expression of different genes involved in the regulation of cell cycling, gene transcription, immunity, and inflammation in their colonic mucosa. A. muciniphila improves wound healing during transmural colonic wall defect through mechanisms possibly involving IL22 signaling and requiring MyD88 in the intestinal cells. In healthy humans, colonic administration of A. muciniphila is well tolerated and changes the expression of genes involved in the immune pathways.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Van Hul, MatthiasMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), UCLouvain, Université Catholique de Louvain, Brussels, Belgium (författare)
  • Baldin, PamelaPathology Department, Cliniques Universitaires Saint-Luc, UCLouvain, Brussels, Belgium (författare)
  • Léonard, DanielColorectal Surgery Unit, Cliniques Universitaires Saint-Luc, Brussels, Belgium (författare)
  • Delzenne, Nathalie M.Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), UCLouvain, Université Catholique de Louvain, Brussels, Belgium (författare)
  • Belzer, ClaraLaboratory of Microbiology, Wageningen University, Wageningen, The Netherlands (författare)
  • Ouwerkerk, Janneke P.Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands (författare)
  • Repsilber, Dirk,1971-Örebro universitet,Institutionen för medicinska vetenskaper,Nutrition-Gut-Brain Interactions Research Centre(Swepub:oru)drr (författare)
  • Rangel, Ignacio,1969-Örebro universitet,Institutionen för medicinska vetenskaper,Nutrition-Gut-Brain Interactions Research Centre(Swepub:oru)irl (författare)
  • Kartheuser, AlexColorectal Surgery Unit, Cliniques Universitaires Saint-Luc, Brussels, Belgium (författare)
  • Brummer, Robert Jan,1957-Örebro universitet,Institutionen för medicinska vetenskaper,Nutrition-Gut-Brain Interactions Research Centre(Swepub:oru)rnbr (författare)
  • De Vos, Willem M.Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands; Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland (författare)
  • Cani, Patrice D.Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), UCLouvain, Université Catholique de Louvain, Brussels, Belgium (författare)
  • Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), UCLouvain, Université Catholique de Louvain, Brussels, Belgium; Colorectal Surgery Unit, Cliniques Universitaires Saint-Luc, Brussels, BelgiumMetabolism and Nutrition Research Group, Louvain Drug Research Institute, Walloon Excellence in Life Sciences and BIOtechnology (WELBIO), UCLouvain, Université Catholique de Louvain, Brussels, Belgium (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Cells: MDPI11:172073-4409

Internetlänk

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  • Cells (Sök värdpublikationen i LIBRIS)

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