A Randomized Trial of the Effect of a GnRH Analogue Injection on Ghrelin Levels in Girls

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Rodanaki, Maria,1987-Örebro universitet,Institutionen för medicinska vetenskaper,Department of Paediatrics (författare)

A Randomized Trial of the Effect of a GnRH Analogue Injection on Ghrelin Levels in Girls

Artikel/kapitelEngelska2022

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2022-07-27
S. Karger,2022
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LIBRIS-ID:oai:DiVA.org:oru-102574
https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-102574URI
https://doi.org/10.1159/000526147DOI

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Språk:engelska
Sammanfattning på:engelska

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Funding agencies:Research Committee and ALF funding, Region Örebro County, SwedenRegional Research Council Mid Sweden
Introduction: Ghrelin concentrations decline during puberty by an unclear mechanism. Acylated ghrelin (AG) is unstable in sampling tubes, but no standardized sampling protocol exists. We hypothesized that ghrelin levels decrease as a consequence of increased gonadotropin-releasing hormone (GnRH) signalling and that the addition of a protease inhibitor to sampling tubes preserves the AG levels.Methods: In this randomized, placebo-controlled, cross-over study, 13 girls with suspected central precocious puberty were included. They performed an adjusted GnRH stimulation test twice and were given Relefact LHRH (R)(100 mu g/m(2)) or saline in a randomized order. Blood was sampled repeatedly for 150 min for the analysis of hormone concentrations. Oestradiol levels were only measured at baseline. The protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) was added to the sampling tubes. Specific ELISA kits were used for the analysis of AG and desacylated ghrelin (DAG) levels.Results: Neither AG nor DAG levels changed after GnRH analogue injection in comparison to saline. The addition of AEBSF preserved AG levels (650.1 +/- 257.1 vs. 247.6 +/- 123.4 pg/mL, p < 0.001) and decreased DAG levels (51.9 [12.5-115.7] vs. 143.5 [71.4-285.7] pg/mL, p < 0.001). Both AG and DAG levels were inversely associated with insulin levels (r = -0.73, p = 0.005, and r = -0.78, p = 0.002, respectively). AG levels were inversely associated with oestradiol levels (rho = -0.57, p = 0.041).Conclusion: Ghrelin levels do not decrease following a pharmacological dose of a GnRH analogue in the short term in girls. Addition of a protease inhibitor to the sampling tubes decreases AG degradation, resulting in preserved AG and decreased DAG levels. (C) 2022 The Author(s). Published by S. Karger AG, Basel

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Pediatrik hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Pediatrics hsv//eng
Acylated ghrelin
Central precocious puberty
Desacylated ghrelin
GnRH analogue
Protease inhibitor

Biuppslag (personer, institutioner, konferenser, titlar ...)

Rask, Eva,1958-Örebro universitet,Institutionen för medicinska vetenskaper,University Health Care Research Centre(Swepub:oru)erk (författare)
Lodefalk, Maria,1968-Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Paediatrics; University Health Care Research Centre(Swepub:oru)malk (författare)
Örebro universitetInstitutionen för medicinska vetenskaper (creator_code:org_t)

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Ingår i:Hormone Research in Paediatrics: S. Karger95:5, s. 442-4511663-28181663-2826

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Av författaren/redakt...: Rodanaki, Maria, ...; Rask, Eva, 1958-; Lodefalk, Maria, ...

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Endokrinologi oc ...

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Pediatrik

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Av lärosätet: Örebro universitet

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