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A Randomized Trial ...
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Rodanaki, Maria,1987-Örebro universitet,Institutionen för medicinska vetenskaper,Department of Paediatrics
(författare)
A Randomized Trial of the Effect of a GnRH Analogue Injection on Ghrelin Levels in Girls
- Artikel/kapitelEngelska2022
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2022-07-27
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S. Karger,2022
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LIBRIS-ID:oai:DiVA.org:oru-102574
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-102574URI
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https://doi.org/10.1159/000526147DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Funding agencies:Research Committee and ALF funding, Region Örebro County, SwedenRegional Research Council Mid Sweden
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Introduction: Ghrelin concentrations decline during puberty by an unclear mechanism. Acylated ghrelin (AG) is unstable in sampling tubes, but no standardized sampling protocol exists. We hypothesized that ghrelin levels decrease as a consequence of increased gonadotropin-releasing hormone (GnRH) signalling and that the addition of a protease inhibitor to sampling tubes preserves the AG levels.Methods: In this randomized, placebo-controlled, cross-over study, 13 girls with suspected central precocious puberty were included. They performed an adjusted GnRH stimulation test twice and were given Relefact LHRH (R)(100 mu g/m(2)) or saline in a randomized order. Blood was sampled repeatedly for 150 min for the analysis of hormone concentrations. Oestradiol levels were only measured at baseline. The protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) was added to the sampling tubes. Specific ELISA kits were used for the analysis of AG and desacylated ghrelin (DAG) levels.Results: Neither AG nor DAG levels changed after GnRH analogue injection in comparison to saline. The addition of AEBSF preserved AG levels (650.1 +/- 257.1 vs. 247.6 +/- 123.4 pg/mL, p < 0.001) and decreased DAG levels (51.9 [12.5-115.7] vs. 143.5 [71.4-285.7] pg/mL, p < 0.001). Both AG and DAG levels were inversely associated with insulin levels (r = -0.73, p = 0.005, and r = -0.78, p = 0.002, respectively). AG levels were inversely associated with oestradiol levels (rho = -0.57, p = 0.041).Conclusion: Ghrelin levels do not decrease following a pharmacological dose of a GnRH analogue in the short term in girls. Addition of a protease inhibitor to the sampling tubes decreases AG degradation, resulting in preserved AG and decreased DAG levels. (C) 2022 The Author(s). Published by S. Karger AG, Basel
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Rask, Eva,1958-Örebro universitet,Institutionen för medicinska vetenskaper,University Health Care Research Centre(Swepub:oru)erk
(författare)
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Lodefalk, Maria,1968-Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Paediatrics; University Health Care Research Centre(Swepub:oru)malk
(författare)
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Örebro universitetInstitutionen för medicinska vetenskaper
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Hormone Research in Paediatrics: S. Karger95:5, s. 442-4511663-28181663-2826
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