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In Vitro Fermentati...
In Vitro Fermentation of Pleurotus eryngii Mushrooms by Human Fecal Microbiota : Metataxonomic Analysis and Metabolomic Profiling of Fermentation Products
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- Christodoulou, Paris (författare)
- National Hellenic Research Foundation, Institute of Chemical Biology, 11635 Athens, Greece; Department of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, Greece
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- Vlassopoulou, Marigoula (författare)
- National Hellenic Research Foundation, Institute of Chemical Biology, 11635 Athens, Greece; Department of Nutrition and Dietetics, Harokopio University, 17676 Kalithea, Greece
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- Zervou, Maria (författare)
- National Hellenic Research Foundation, Institute of Chemical Biology, 11635 Athens, Greece
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- Xanthakos, Evangelos (författare)
- National Hellenic Research Foundation, Institute of Chemical Biology, 11635 Athens, Greece; Department of Biotechnology, Agricultural University of Athens, 11855 Athens, Greece
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- Moulos, Panagiotis (författare)
- Biomedical Sciences Research Center Alexander Fleming, 16672 Vari, Greece
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- Koutrotsios, Georgios (författare)
- Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, 11855 Athens, Greece
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- Zervakis, Georgios I. (författare)
- Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, 11855 Athens, Greece
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- Kerezoudi, Evangelia N., 1995- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,Department of Nutrition and Dietetics, Harokopio University, 17676 Kalithea, Greece
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- Mitsou, Evdokia K. (författare)
- Department of Nutrition and Dietetics, Harokopio University, 17676 Kalithea, Greece
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- Saxami, Georgia (författare)
- Department of Nutrition and Dietetics, Harokopio University, 17676 Kalithea, Greece
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- Kyriacou, Adamantini (författare)
- Department of Nutrition and Dietetics, Harokopio University, 17676 Kalithea, Greece
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- Pletsa, Vasiliki (författare)
- National Hellenic Research Foundation, Institute of Chemical Biology, 11635 Athens, Greece
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- Georgiadis, Panagiotis (författare)
- National Hellenic Research Foundation, Institute of Chemical Biology, 11635 Athens, Greece
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(creator_code:org_t)
- 2023-01-16
- 2023
- Engelska.
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Ingår i: Journal of fungi (Basel, Switzerland). - : MDPI. - 2309-608X. ; 9:1
- Relaterad länk:
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Edible mushrooms contain biologically active compounds with antioxidant, antimicrobial, immunomodulatory and anticancer properties. The link between their anticancer and immunomodulatory properties with their possible prebiotic activity on gut micro-organisms has been the subject of intense research over the last decade. Lyophilized Pleurotus eryngii (PE) mushrooms, selected due to their strong lactogenic effect and anti-genotoxic, immunomodulatory properties, underwent in vitro static batch fermentation for 24 h by fecal microbiota from eight elderly apparently healthy volunteers (>65 years old). The fermentation-induced changes in fecal microbiota communities were examined using Next Generation Sequencing of the hypervariable regions of the 16S rRNA gene. Primary processing and analysis were conducted using the Ion Reporter Suite. Changes in the global metabolic profile were assessed by 1H NMR spectroscopy, and metabolites were assigned by 2D NMR spectroscopy and the MetaboMiner platform. PLS-DA analysis of both metataxonomic and metabolomic data showed a significant cluster separation of PE fermented samples relative to controls. DEseq2 analysis showed that the abundance of families such as Lactobacillaceae and Bifidobacteriaceae were increased in PE samples. Accordingly, in metabolomics, more than twenty metabolites including SCFAs, essential amino acids, and neurotransmitters discriminate PE samples from the respective controls, further validating the metataxonomic findings.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Pleurotus eryngii mushrooms
- gut microbiota
- in vitro static batch fermentation
- metabolomics
- metataxonomics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Christodoulou, P ...
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Vlassopoulou, Ma ...
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Zervou, Maria
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Xanthakos, Evang ...
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Moulos, Panagiot ...
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Koutrotsios, Geo ...
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Zervakis, Georgi ...
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Kerezoudi, Evang ...
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Mitsou, Evdokia ...
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Saxami, Georgia
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Kyriacou, Adaman ...
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Pletsa, Vasiliki
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Georgiadis, Pana ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
- Artiklar i publikationen
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Journal of fungi ...
- Av lärosätet
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Örebro universitet