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Genotypic character...
Genotypic characterization of Staphylococcus chromogenes and Staphylococcus simulans from Swedish cases of bovine subclinical mastitis
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- Waller, K. Persson (author)
- Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE-751 89 Uppsala, Sweden
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- Myrenås, M. (author)
- Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE-751 89 Uppsala, Sweden
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- Börjesson, Stefan, 1979- (author)
- Örebro universitet,Institutionen för hälsovetenskaper,School of Health Science, Örebro University, Örebro, Sweden
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- Kim, H. (author)
- Department of Disease Control and Epidemiology, National Veterinary Institute (SVA), SE-751 89 Uppsala, Sweden
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- Widerström, Micael, 1958- (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Monsen, Tor J. (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Sigurðarson Sandholt, A K (author)
- deCODE genetics, Sturlugata 8, Reykjavik 101, Iceland
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- Östlund, E. (author)
- Department of Microbiology, National Veterinary Institute (SVA), SE-75189 Uppsala, Sweden
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- Cha, W. (author)
- Department of Disease Control and Epidemiology, National Veterinary Institute (SVA), SE-751 89 Uppsala, Sweden
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(creator_code:org_t)
- American Dairy Science Association, 2023
- 2023
- English.
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In: Journal of Dairy Science. - : American Dairy Science Association. - 0022-0302 .- 1525-3198. ; 106:11, s. 7991-8004
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Abstract
Subject headings
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- Staphylococcus chromogenes and Staphylococcus simulans are commonly found in intramammary infections (IMI) associated with bovine subclinical mastitis, but little is known about genotypic variation and relatedness within species. This includes knowledge about genes encoding antimicrobial resistance (AMR) and potential virulence factors (pVF). The aim of this study was therefore to investigate these aspects by whole-genome sequencing of milk isolates from Swedish dairy cows with subclinical mastitis in an observational study. We also wanted to study if specific genotypes were associated with persistent IMI and the inflammatory response at udder quarter level. In total, 105 and 118 isolates of S. chromogenes and S. simulans, respectively, were included. Isolates were characterized using a 7-locus multilocus sequence typing (7-MLST), core genome analysis and in-silico analysis of AMR and pVF genes. Forty-seven sequence types (ST) and 7 core genome clusters of S. chromogenes were identified, and the most common ST were ST-6 and ST-109, both belonging to cluster VII. A 7-locus MLST scheme for S. simulans was not available, but 3 core genome clusters and 5 subclusters were described. Overall, substantial variation in ST and clusters among cows and herds were found in both species. Some ST of S. chromogenes were found in several herds, indicating spread between herds. Moreover, within-herd spread of the same genotype was observed for both species. Only a few AMR genes (blaZ, strpS194, vga(A)) were detected in a limited number of isolates, with the exception of blaZ coding for β-lactamase, which was identified in 22% of the isolates of S. chromogenes with ST-19, ST-102, and ST-103 more commonly carrying this gene compared with other STs. However, the blaZ gene was not identified in S. simulans. The average total number of pVF detected per isolate was similar in S. chromogenes (n = 30) and S. simulans (n = 33), but some variation in total numbers and presence of specific pVF or functional groups of pVF, was shown between ST/clusters within species. Differences in inflammatory response and potentially in persistent IMI at udder quarter level were found between S. chromogenes subtypes but not between S. simulans subtypes. In conclusion, the results from the present study generates new insight into the epidemiology of bovine S. chromogenes and S. simulans IMI, which can have implications for future prevention and antimicrobial treatment of infections related to these species.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Keyword
- Antimicrobial resistance
- multilocus sequence typing
- nonaureus staphylococci
- virulence factors
- whole-genome sequencing
Publication and Content Type
- ref (subject category)
- art (subject category)
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