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Pyrosequencing assa...
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Böttiger, A. K.Örebro universitet,Hälsoakademin
(author)
Pyrosequencing assay for genotyping of the Transcobalamin II 776C>G polymorphism
- Article/chapterEnglish2007
Publisher, publication year, extent ...
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2009-07-08
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Oslo :Taylor & Francis,2007
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:oru-11545
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-11545URI
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https://doi.org/10.1080/00365510601026542DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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The 776C>G polymorphism of the Transcobalamin II gene is located in a GC-rich region and TaqMan real-time polymerase chain reaction (PCR) does not yield satisfactory genotyping results. We therefore hypothesized that a method based on DNA sequencing would be needed for this single nucleotide polymorphism (SNP) analysis; Pyrosequencing technology was tested for this purpose. A Pyrosequencing protocol was developed, optimized and applied to a sample of 389 Swedish senior citizens. The three genotypes CC, CG, and GG consistently yielded typical programs that were readily distinguishable from each other. The prevalence of the mutated allele in the studied Swedish population was q=0.445. It is concluded that the TC II 776C>G polymorphism can be reliably genotyped by Pyrosequencing technology.
Subject headings and genre
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MEDICINE
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MEDICIN
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Medicine
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medicin
Added entries (persons, corporate bodies, meetings, titles ...)
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Nilsson, Torbjörn K.Örebro universitet,Hälsoakademin(Swepub:oru)tnnn
(author)
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Örebro universitetHälsoakademin
(creator_code:org_t)
Related titles
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In:Scandinavian Journal of Clinical and Laboratory InvestigationOslo : Taylor & Francis67:2, s. 247-2510036-55131502-7686
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