Regulation of human epithelial tight junction proteins by Lactobacillus plantarum in vivo and protective effects on the epithelial barrier

Karczewski, JurgenHost Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands (författare)

Lactobacillus plantarum, a commensal bacterium of humans, has been proposed to enhance the intestinal barrier, which is compromised in a number of intestinal disorders. To study the effect of L. plantarum strain WCFS1 on human barrier function, healthy subjects were administered L. plantarum or placebo in the duodenum for 6 h by means of a feeding catheter. The scaffold protein zonula occludens (ZO)-1 and transmembrane protein occludin were found to be significantly increased in the vicinity of the tight-junction (TJ) structures, which form the paracellular seal between cells of the epithelium. In an in vitro model of the human epithelium, L. plantarum induced translocation of ZO-1 to the TJ region; however, the effects on occludin were minor compared with those seen in vivo. L. plantarum was shown to activate Toll-like receptor 2 (TLR2) signaling, and treatment of Caco-2 monolayers with the TLR2 agonist Pam3-Cys-SK4(PCSK) significantly increased fluorescent staining of occludin in the TJ. Pretreatment of Caco-2 monolayers with L. plantarum or PCSK significantly attenuated the effects of phorbol ester-induced dislocation of ZO-1 and occludin and the associated increase in epithelial permeability. Our results identifying commensal bacterial stimulation of TLR2 in the gut epithelium as a regulator of epithelial integrity have important implications for understanding probiotic mechanisms and the control of intestinal homeostasis.

Troost, Freddy J.TI Food & Nutr, Wageningen, Netherlands; Div Gastroenterol & Hepatol, Dept Internal Med, Nutr & Toxicol Res Inst Maastricht, Maastricht Univ, Maastricht, Netherlands (författare)
Konings, IreneHost Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands (författare)
Dekker, JanTI Food & Nutr, Wageningen, Netherlands (författare)
Kleerebezem, MichielTI Food & Nutr, Wageningen, Netherlands; NIZO Food Res, Ede, Netherlands; Microbiol Lab, Univ Wageningen, Wageningen, Netherlands (författare)
Brummer, RobertÖrebro universitet,Hälsoakademin,TI Food & Nutr, Wageningen, Netherlands; Div Gastroenterol & Hepatol, Dept Internal Med, Nutr & Toxicol Res Inst Maastricht, Maastricht Univ, Maastricht, Netherlands(Swepub:oru)rnbr (författare)
Wells, Jerry M.Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, Netherlands (författare)
Host Microbe Interact Grp, Univ Wageningen, Wageningen, Netherlands; TI Food & Nutr, Wageningen, NetherlandsTI Food & Nutr, Wageningen, Netherlands; Div Gastroenterol & Hepatol, Dept Internal Med, Nutr & Toxicol Res Inst Maastricht, Maastricht Univ, Maastricht, Netherlands (creator_code:org_t)

Ingår i:American Journal of Physiology - Gastrointestinal and Liver Physiology: American Physiological Society298:6, s. G851-G8590193-18571522-1547

Av författaren/redakt...: Karczewski, Jurg ...; Troost, Freddy J ...; Konings, Irene; Dekker, Jan; Kleerebezem, Mic ...; Brummer, Robert; visa fler...; Wells, Jerry M.; visa färre...

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