Elution of antitransglutaminase antibodies from duodenal biopsies: a novel approach in the diagnosis of celiac disease

Rathsman, SandraDepartment of Laboratory Medicine/Microbiology, Örebro University Hospital, Örebro, Sweden (författare)

Elution of antitransglutaminase antibodies from duodenal biopsies : a novel approach in the diagnosis of celiac disease

Celiac disease (CeD) is a disease more prevalent and multisymptomatic than was earlier recognized. Whereas prompt initiation of gluten-free diet (GFD) is beneficial in relieving the symptoms, an accurate CeD diagnosis is necessary also to avoid years of restricted diet on uncertain grounds. We propose a new diagnostic method, based on elution of deposited antibodies against transglutaminase (anti-tTG) from duodenal biopsies in patients with symptoms and screening serology analyses suggestive of CeD. The eluates were analyzed in a Phadia 250 fluoroimmunoassay, demonstrating elevated concentrations of anti-tTG in CeD patients, corresponding to serology and histopathology findings. In one case histology was inconclusive, displaying only unspecific inflammation, but eluted anti-tTG was positive. This patient has clinically improved following GFD. We conclude that our novel method represents a new tool in the diagnostic work up in CeD. The detection of deposited anti-tTG at the site of inflammation appears to provide a high sensitivity and specificity using a technique that is quick, simple and reliable. Further studies are needed for optimization and elucidation of suitable applications for this elution method.

Tysk, Curt,1949-Örebro universitet,Institutionen för hälsovetenskap och medicin,Division of Gastroenterology, Department of Medicine, Örebro University Hospital, Örebro, Sweden(Swepub:oru)ctk (författare)
Eriksson, SuneDepartment of Laboratory Medicine/Pathology, Örebro University Hospital, Örebro, Sweden (författare)
Hultgren, Olof,1970-Örebro universitet,Institutionen för hälsovetenskap och medicin,Department of Laboratory Medicine/Microbiology, Örebro University Hospital, Örebro, Sweden(Swepub:oru)ovhn (författare)
Åberg, Anna-KarinDepartment of Laboratory Medicine/Microbiology, Örebro University Hospital, Örebro, Sweden (författare)
Olcén, PerDepartment of Laboratory Medicine/Microbiology, Örebro University Hospital, Örebro, Sweden (författare)
Department of Laboratory Medicine/Microbiology, Örebro University Hospital, Örebro, SwedenInstitutionen för hälsovetenskap och medicin (creator_code:org_t)

Ingår i:Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)Hoboken, USA : Wiley-Blackwell120:8, s. 666-6740903-46411600-0463

Av författaren/redakt...: Rathsman, Sandra; Tysk, Curt, 1949 ...; Eriksson, Sune; Hultgren, Olof, ...; Åberg, Anna-Kari ...; Olcén, Per

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