Skin barrier disruption by sodium lauryl sulfate-exposure alters the expressions of involucrin, transglutaminase 1, profilaggrin, and kallikreins during the repair phase in human skin in vivo

• Detergents are skin irritants affecting keratinocytes. In this study, healthy volunteers were exposed to water (vehicle) and 1% sodium lauryl sulfate (SLS) under occlusive patch tests for 24 hours. The messenger RNA (mRNA) expression of keratinocyte differentiation markers and of enzymes involved in corneodesmosome degradation was examined in skin biopsies (n=8) during the repair phase (6 hours to 7 days postexposure) using real-time reverse-transcription PCR. It was found that the expression of involucrin was increased at 6 hours, but then rapidly normalized. The expression of transglutaminase 1 exhibited a twofold increase after 24 hours in the SLS-exposed skin. Profilaggrin was decreased after 6 hours. Later (4–7 days), the expression in SLS-exposed areas was >50% above than in control areas. An increased and altered immunofluorescence pattern of involucrin, transglutaminase 1, and filaggrin was also found (n=4). At 6 hours post-SLS exposure, the mRNA expression of kallikrein-7 (KLK-7) and kallikrein-5 (KLK-5) was decreased by 50 and 75%, respectively, as compared with control and water-exposed areas. Thereafter, the expression pattern of KLK-7 and KLK-5 was normalized. Changes in protein expression of KLK-5 were also found. In conclusion, SLS-induced skin barrier defects induce altered mRNA expression of keratinocyte differentiation markers and enzymes degrading corneodesmosomes.

Ämnesord

NATURVETENSKAP  -- Matematik (hsv//swe)

NATURAL SCIENCES  -- Mathematics (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)

Nyckelord

Adult

Biopsy

Cell Differentiation/drug effects

Dermatitis; Irritant/pathology/*physiopathology

Female

Humans

Intermediate Filament Proteins/*genetics/metabolism

Irritants/toxicity

Kallikreins/*genetics/metabolism

Keratinocytes/drug effects/metabolism/pathology

Male

Middle Aged

Patch Tests

Protein Precursors/*genetics/metabolism

RNA; Messenger/metabolism

Reverse Transcriptase Polymerase Chain Reaction

Skin/metabolism/pathology/physiopathology

Sodium Dodecyl Sulfate/toxicity

Transglutaminases/*genetics/metabolism

Wound Healing/physiology

MATHEMATICS

MATEMATIK

Dermatology and venerology

Dermatologi och venerologi

Dermatology and Venerology

Dermatologi och venereologi

MEDICINE

Publikations- och innehållstyp

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