Skin barrier disruption by sodium lauryl sulfate-exposure alters the expressions of involucrin, transglutaminase 1, profilaggrin, and kallikreins during the repair phase in human skin in vivo

Detergents are skin irritants affecting keratinocytes. In this study, healthy volunteers were exposed to water (vehicle) and 1% sodium lauryl sulfate (SLS) under occlusive patch tests for 24 hours. The messenger RNA (mRNA) expression of keratinocyte differentiation markers and of enzymes involved in corneodesmosome degradation was examined in skin biopsies (n=8) during the repair phase (6 hours to 7 days postexposure) using real-time reverse-transcription PCR. It was found that the expression of involucrin was increased at 6 hours, but then rapidly normalized. The expression of transglutaminase 1 exhibited a twofold increase after 24 hours in the SLS-exposed skin. Profilaggrin was decreased after 6 hours. Later (4–7 days), the expression in SLS-exposed areas was >50% above than in control areas. An increased and altered immunofluorescence pattern of involucrin, transglutaminase 1, and filaggrin was also found (n=4). At 6 hours post-SLS exposure, the mRNA expression of kallikrein-7 (KLK-7) and kallikrein-5 (KLK-5) was decreased by 50 and 75%, respectively, as compared with control and water-exposed areas. Thereafter, the expression pattern of KLK-7 and KLK-5 was normalized. Changes in protein expression of KLK-5 were also found. In conclusion, SLS-induced skin barrier defects induce altered mRNA expression of keratinocyte differentiation markers and enzymes degrading corneodesmosomes.

NATURVETENSKAP Matematik hsv//swe
NATURAL SCIENCES Mathematics hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Dermatologi och venereologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dermatology and Venereal Diseases hsv//eng
Adult
Biopsy
Cell Differentiation/drug effects
Dermatitis; Irritant/pathology/*physiopathology
Female
Humans
Intermediate Filament Proteins/*genetics/metabolism
Irritants/toxicity
Kallikreins/*genetics/metabolism
Keratinocytes/drug effects/metabolism/pathology
Male
Middle Aged
Patch Tests
Protein Precursors/*genetics/metabolism
RNA; Messenger/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Skin/metabolism/pathology/physiopathology
Sodium Dodecyl Sulfate/toxicity
Transglutaminases/*genetics/metabolism
Wound Healing/physiology
MATHEMATICS
MATEMATIK
Dermatology and venerology
Dermatologi och venerologi
Dermatology and Venerology
Dermatologi och venereologi
MEDICINE

Lindberg, MagnusKarolinska Institutet,Örebro universitet,Hälsoakademin(Swepub:oru)mslg (author)
Berne, BeritUppsala universitet,Dermatologi och venereologi(Swepub:uu)beribern (author)
Uppsala universitetDermatologi och venereologi (creator_code:org_t)

In:Journal of Investigative DermatologyLondon : Nature publishing group128:5, s. 1212-12190022-202X1523-1747

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Dermatology and ...

By the university: Örebro University; Uppsala University; Karolinska Institutet

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