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IL-8 and global gen...
IL-8 and global gene expression analysis define a key role of ATP in renal epithelial cell responses induced by uropathogenic bacteria
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- Kruse, Robert, 1972- (författare)
- Örebro universitet,Institutionen för läkarutbildning,Region Örebro län,Faculty of Medicine and Health, Örebro University, Örebro, Sweden; Clinical Research Centre (KFC), Örebro University Hospital, Örebro, Sweden,iRiSC — Inflammatory Response and Infection Susceptibility Centre,Univ Örebro
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- Demirel, Isak, 1987- (författare)
- Örebro universitet,Institutionen för hälsovetenskap och medicin,Univ Örebro
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- Säve, Susanne (författare)
- Linnéuniversitetet,Institutionen för kemi och biomedicin (KOB)
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- Persson, Katarina, 1962- (författare)
- Örebro universitet,Institutionen för läkarutbildning,Faculty of Medicine and Health, Örebro University, Örebro, Sweden,iRiSC — Inflammatory Response and Infection Susceptibility Centre,Univ Örebro
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(creator_code:org_t)
- 2014-05-10
- 2014
- Engelska.
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Ingår i: Purinergic Signalling Purinergic Signalling. - Dordrect, Netherlands : Springer. - 1573-9538 .- 1573-9546. ; 10:3, s. 499-508
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- The recent recognition of receptor-mediated ATP signalling as a pathway of epithelial pro-inflammatory cytokine release challenges the ubiquitous role of the TLR4 pathway during urinary tract infection. The aim of this study was to compare cellular responses of renal epithelial cells infected with uropathogenic Escherichia coli (UPEC) strain IA2 to stimulation with ATP-gamma-S. A498 cells were infected or stimulated in the presence or absence of apyrase, that degrades extracellular ATP, or after siRNA-mediated knockdown of ATP-responding P2Y(2) receptors. Cellular IL-8 release and global gene expression were analysed. Both IA2 and A498 cells per se released ATP, which increased during infection. IA2 and ATP-gamma-S caused a similar to 5-fold increase in cellular release of IL-8 and stimulations performed in the presence of apyrase or after siRNA knockdown of P2Y(2) receptors resulted in attenuation of IA2-mediated IL-8 release. Microarray results show that both IA2 and ATP-gamma-S induced marked changes in gene expression of renal cells. Thirty-six genes were in common between both stimuli, and many of these are key genes belonging to classical response pathways of bacterial infection. Functional analysis shows that 88 biological function-annotated cellular pathways were identical between IA2 and ATP-gamma-S stimuli. Results show that UPEC-induced release of IL-8 is dependent on P2Y(2) signalling and that cellular responses elicited by UPEC and ATP-gamma-S have many identical features. This indicates that renal epithelial responses elicited by bacteria could be mediated by bacteria- or host-derived ATP, thus defining a key role of ATP during infection.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Urinary tract infection
- Host response
- Adenosine triphosphate
- Uropathogenic E. coli
- Purinergic P2Y receptors
- Neurology
- Neurologi
- Molecular Biology
- Molekylärbiologi
- Biomedicinsk vetenskap
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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