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Supplementary ultra...
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Kalbina, IrinaÖrebro universitet,Institutionen för naturvetenskap,Molekylär biokemi
(författare)
Supplementary ultraviolet-B irradiation reveals differences in stress responses between Arabidopsis thaliana ecotypes
- Artikel/kapitelEngelska2006
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Wiley,2006
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LIBRIS-ID:oai:DiVA.org:oru-4133
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-4133URI
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https://doi.org/10.1111/j.1365-3040.2005.01436.xDOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Irradiation of Arabidopsis thaliana ecotypes C24, Wassilewskija (Ws) and Columbia-0 (Col-0) with supplementary ultraviolet-A+B (UV-A+B) radiation revealed ecotype-specific differences in expression of the gene for the pathogenesis-related protein PR-5. C24 showed an increased expression level of PR-5 (5- and 20-fold higher compared with Col-0 and Ws, respectively). Expression of other molecular markers such as CHS (encoding chalcone synthase), MEB5.2 [encoding a gene strongly up-regulated by ultraviolet-B (UV-B)] and PYROA [encoding a pyridoxine (Vitamin B6) biosynthesis enzyme] only showed slight differences between ecotypes. Oxidative stress during UVA+B exposure was monitored by staining for H2O2. This analysis also revealed important ecotype-specific differences. 'H2O2 hot spots' were found in C24, whereas an even distribution of H2O2 was found in Ws and Col-0. Necrotic lesions also appeared on C24 leaves after prolonged UV-B exposure. There was a reverse correlation between the H2O2 steady-state concentration and the PR-5 gene expression; Ws showed the highest level of H2O2 accumulation but the lowest expression level of the PR-5 gene. Furthermore, application of paraquat on the rosettes led to similar PR-5 expression and H2O2 accumulation patterns as were found after UV-A+B irradiation. The observed ecotypic differences were also reflected in a statistically significant UV-B-dependent decrease in biomass, rosette size and leaf area for Ws, but not for C24 and Col-0. Our results show that a significant ecotype-specific genetic variability in general UV-B responses in Arabidopsis exists. Moreover, the signal transduction or gene regulation pathway for PR-5 differs from the other molecular markers used in this study.
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Strid, ÅkeÖrebro universitet,Institutionen för naturvetenskap,Molekylär biokemi(Swepub:oru)aesd
(författare)
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Örebro universitetInstitutionen för naturvetenskap
(creator_code:org_t)
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Ingår i:Plant, Cell and Environment: Wiley29:5, s. 754-7630140-77911365-3040
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