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Rapid detection of Panton-Valentine leukocidin in Staphylococcus aureus cultures by use of a lateral flow assay based on monoclonal antibodies
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- Monecke, Stefan (author)
- Alere Technologies GmbH, Jena, Germany; Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany
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- Müller, Elke (author)
- Alere Technologies GmbH, Jena, Germany
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- Buechler, Joseph (author)
- Alere San Diego, Inc., San Diego CA, United States
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- Rejman, John (author)
- Alere Scarborough Inc., Scarborough ME, United States
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- Stieber, Bettina (author)
- Alere Technologies GmbH, Jena, Germany; Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany
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- Akpaka, Patrick Eberechi (author)
- Department of Para-Clinical Sciences, University of West Indies, St. Augustine, Trinidad and Tobago
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- Bandt, Dirk (author)
- Institute for Medical Diagnostics Oderland, Frankfurt an der Oder, Germany
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- Burris, Rob (author)
- Alere Technologies GmbH, Entebbe, Uganda
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- Coombs, Geoffrey (author)
- Australian Collaborat. Cen. for Enterococcus and Staphylococcus Species (ACCESS) Typing and Res., Curtin University WA, Perth WA, Australia; Royal Perth Hospital, Perth WA, Australia
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- Hidalgo-Arroyo, G Aida (author)
- Bristol Centre for Antimicrobial Research and Evaluation, Southmead Hospital, Bristol, United Kingdom
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- Hughes, Peter (author)
- Medical Research Council, Entebbe, Uganda
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- Kearns, Angela (author)
- Staphylococcus Reference Unit, Microbiology Services-Colindale, Health Protection Agency, London, United Kingdom
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- Abós, Sonia Molinos (author)
- Hospital Universitari Germans Trias i Pujol, Badalona, Spain; Universitat Autonoma de Barcelona, Barcelona, Spain
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- Pichon, Bruno (author)
- Staphylococcus Reference Unit, Microbiology Services-Colindale, Health Protection Agency, London, United Kingdom
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- Skakni, Leila (author)
- Molecular Pathology Laboratory, King Fahad Medical City, Riyadh, Saudi Arabia
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- Söderquist, Bo, 1955- (author)
- Region Örebro län,Departments of Laboratory Medicine and Clinical Microbiology
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- Ehricht, Ralf (author)
- Alere Technologies GmbH, Jena, Germany
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(creator_code:org_t)
- Washington, USA : American society of microbiology, 2013
- 2013
- English.
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In: Journal of Clinical Microbiology. - Washington, USA : American society of microbiology. - 0095-1137 .- 1098-660X. ; 51:2, s. 487-95
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spotted on protein microarrays and screened using different lukF-PV preparations and detection antibodies. This led to the identification of the optimal antibody combination that was then used to establish a lateral flow assay. This test was used to detect PVL in S. aureus cultures. The detection limit of the assay with purified native and recombinant antigens was determined to be around 1 ng/ml. Overnight cultures from various solid and liquid media proved suitable for PVL detection. Six hundred strains and clinical isolates from patients from America, Europe, Australia, Africa, and the Middle East were tested. Isolates were genotyped in parallel by DNA microarray hybridization for confirmation of PVL status and assignment to clonal complexes. The sensitivity, specificity, and positive and negative predictive values of the assay in this trial were 99.7, 98.3, 98.4, and 99.7%, respectively. A total of 302 clinical isolates and reference strains were PVL positive and were assigned to 21 different clonal complexes. In summary, the lateral flow test allows rapid and economical detection of PVL in a routine bacteriology laboratory. As the test utilizes cultures from standard media and does not require sophisticated equipment, it can be easily integrated into a laboratory's workflow and might contribute to timely therapy of PVL-associated infections.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Monecke, Stefan
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Müller, Elke
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Buechler, Joseph
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Rejman, John
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Stieber, Bettina
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Akpaka, Patrick ...
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Bandt, Dirk
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Burris, Rob
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Coombs, Geoffrey
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Hidalgo-Arroyo, ...
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Hughes, Peter
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Kearns, Angela
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Abós, Sonia Moli ...
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Pichon, Bruno
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Skakni, Leila
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Söderquist, Bo, ...
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Ehricht, Ralf
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Clinical Laborat ...
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Örebro University