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Division of Listeri...
Division of Listeria monocytogenes serovar 1/2a strains into two groups by PCR and restriction enzyme analysis
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- Unnerstad, Helle (author)
- Department of Food Hygiene, Faculty of Veterinary Medicine, Sweden University of Agricultural Sciences, (SLU), Uppsala
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- Nilsson, Inger (author)
- Department of Food Hygiene, Faculty of Veterinary Medicine, Sweden University of Agricultural Sciences, (SLU), Uppsala
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- Ericsson, Henrik (author)
- Department of Food Hygiene, Faculty of Veterinary Medicine, Sweden University of Agricultural Sciences(SLU), Uppsala
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- Danielsson-Tham, Marie-Louise, 1947- (author)
- Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden
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- Bille, Jacques (author)
- Medical Bacteriology, University Hospital, Lausanne, Switzerland
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- Bannerman, Elizabeth (author)
- Medical Bacteriology, University Hospital, Lausanne, Switzerland
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- Tham, Wilhelm, 1951- (author)
- Department of Food Hygiene, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala, Sweden
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(creator_code:org_t)
- Washington DC, USA : American Society for Microbiology, 1999
- 1999
- English.
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In: Applied and Environmental Microbiology. - Washington DC, USA : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 65:5, s. 2054-2056
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), and 1,876 bp of the gene inlB, was cleaved with the enzyme AluI, and the fragments generated were separated by gel electrophoresis. By this method two different cleavage patterns were obtained. Seventy of the 100 strains shared one restriction profile, and the remaining 30 strains shared the second one. No relation was found between the types differentiated by PCR-REA and the origins of the strains.
Subject headings
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Family Medicine
- Allmänmedicin
- Microbiology
- Mikrobiologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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