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Signal modelization...
Signal modelization for improved precision of assessment of minimum and mean telomere lengths
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- Ponsot, Elodie, 1973- (författare)
- Örebro universitet,Hälsoakademin,Muscle & Exercise Physiology Research Group , RISPA
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- Kadi, Fawzi, 1970- (författare)
- Örebro universitet,Hälsoakademin,Muscle & Exercise Physiology Research Group , RISPA
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(creator_code:org_t)
- Wiley-VCH Verlagsgesellschaft, 2008
- 2008
- Engelska.
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Ingår i: Electrophoresis. - : Wiley-VCH Verlagsgesellschaft. - 0173-0835 .- 1522-2683. ; 29:2, s. 542-544
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Telomere length is an important measure of cell and tissue regenerative capacities. The mean telomere length is classically used as global indicator of a tissue telomere length. In skeletal muscle, which is made of postmitotic myonuclei and satellite cells (muscle stem cells), minimum telomere length is also used to assess the telomere length of satellite cells and newly incorporated myonuclei. At present, the estimation of the method reproducibility during the assessment of mean and minimum telomere length using Southern blot analysis has never been documented. The aim of this report is to describe a signal modelization for improved precision of assessment of minimum and mean telomere lengths and to document the method reproducibility. Telomeres are assessed using a Southern technique where the gel is directly hybridized with the specific probe without the membrane-transferring step in order to prevent telomeric low signal loss. We found that the improved signal analysis for determination of telomere length is associated with coefficients of variation ranging from 1.37 to 4.29% for the mean telomeric restriction fragment (TRF) length and from 2.04 to 4.95% for the minimum TRF length. Improved method reproducibility would allow saving time and biological material as duplicate and triplicate measurement of the same sample is no longer required.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- SAMHÄLLSVETENSKAP -- Annan samhällsvetenskap -- Tvärvetenskapliga studier inom samhällsvetenskap (hsv//swe)
- SOCIAL SCIENCES -- Other Social Sciences -- Social Sciences Interdisciplinary (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Idrottsvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Sport and Fitness Sciences (hsv//eng)
Nyckelord
- Automatic Data Processing
- Blotting; Southern/methods
- Muscle; Skeletal/chemistry
- Telomere/*chemistry
- Physiology
- Fysiologi
- MEDICINE
- MEDICIN
- INTERDISCIPLINARY RESEARCH AREAS
- TVÄRVETENSKAPLIGA FORSKNINGSOMRÅDEN
- Sports
- Idrott
- Sports Science
- Idrottsvetenskap
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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