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Exopolysaccharide-p...
Exopolysaccharide-producing probiotic Lactobacilli reduce serum cholesterol and modify enteric microbiota in ApoE-deficient mice
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- London, Lis E. E. (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Teagasc Food Research Centre Moorepark, Fermoy, Ireland
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- Kumar, Arun H. S. (author)
- Centre for Research in Vascular Biology, University College Cork, Cork, Ireland
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- Wall, Rebecca, 1979- (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland,Nutrition-Gut-Brain Interactions Research Centre
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- Casey, Pat G. (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland
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- O'Sullivan, Orla (author)
- Teagasc Food Research Centre Moorepark, Fermoy, Ireland
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- Shanahan, Fergus (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland
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- Hill, Colin (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland
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- Cotter, Paul D. (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Teagasc Food Research Centre Moorepark, Fermoy, Ireland
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- Fitzgerald, Gerald F. (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland
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- Ross, R. Paul (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland
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- Caplice, Noel M. (author)
- Centre for Research in Vascular Biology, University College Cork, Cork, Ireland
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- Stanton, Catherine (author)
- Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland; Teagasc Food Research Centre Moorepark, Fermoy, Ireland
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(creator_code:org_t)
- Bethesda : American Society for Nutrition, 2014
- 2014
- English.
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In: Journal of Nutrition. - Bethesda : American Society for Nutrition. - 0022-3166 .- 1541-6100. ; 144:12
- Related links:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
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- Background: Probiotic bacteria have been associated with a reduction in cardiovascular disease risk, a leading cause of death and disability.Objectives: The aim of this study was to assess the impact of dietary administration of exopolysaccharide-producing probiotic Lactobacillus cultures on lipid metabolism and gut microbiota in apolipoprotein E (apoE)-deficient mice.Methods: First, we examined lipid metabolism in response to dietary supplementation with recombinant β-glucan-producing Lactobacillus paracasei National Food Biotechnology Centre (NFBC) 338 expressing the glycosyltransferase (Gtf) gene from Pediococcus parvulus 2.6 (GTF), and naturally exopolysaccharide-producing Lactobacillus mucosae Dairy Product Culture Collection (DPC) 6426 (DPC 6426) compared with the non-β-glucan-producing isogenic control strain Lactobacillus paracasei NFBC 338 (PNZ) and placebo (15% wt:vol trehalose). Second, we examined the effects on the gut microbiota of dietary administration of DPC 6426 compared with placebo. Probiotic Lactobacillus strains at 1 × 10(9) colony-forming units/d per animal were administered to apoE(-/-) mice fed a high-fat (60% fat)/high-cholesterol (2% wt:wt) diet for 12 wk. At the end of the study, aortic plaque development and serum, liver, and fecal variables involved in lipid metabolism were analyzed, and culture-independent microbial analyses of cecal content were performed.Results: Total cholesterol was reduced in serum (P < 0.001; ∼33-50%) and liver (P < 0.05; ∼30%) and serum triglyceride concentrations were reduced (P < 0.05; ∼15-25%) in mice supplemented with GTF or DPC 6426 compared with the PNZ or placebo group, respectively. In addition, dietary intervention with GTF led to increased amounts of fecal cholesterol excretion (P < 0.05) compared with all other groups. Compositional sequencing of the gut microbiota revealed a greater prevalence of Porphyromonadaceae (P = 0.001) and Prevotellaceae (P = 0.001) in the DPC 6426 group and lower proportions of Clostridiaceae (P < 0.05), Peptococcaceae (P < 0.001), and Staphylococcaceae (P < 0.01) compared with the placebo group.Conclusion: Ingestion of exopolysaccharide-producing lactobacilli resulted in seemingly favorable improvements in lipid metabolism, which were associated with changes in the gut microbiota of mice.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Näringslära (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Nutrition and Dietetics (hsv//eng)
Keyword
- probiotics
- lactobacilli
- exopolysaccharide
- cholesterol
- gut microbiota
- lipid metabolism
Publication and Content Type
- ref (subject category)
- art (subject category)
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London, Lis E. E ...
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Kumar, Arun H. S ...
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Wall, Rebecca, 1 ...
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Casey, Pat G.
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O'Sullivan, Orla
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Shanahan, Fergus
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Hill, Colin
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Cotter, Paul D.
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Fitzgerald, Gera ...
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Ross, R. Paul
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Caplice, Noel M.
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Stanton, Catheri ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Nutrition and Di ...
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Journal of Nutri ...
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Örebro University