Sökning: WFRF:(O'Mahony Liam) >
Recombinant lactoba...
Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice
-
- Rosberg-Cody, Eva (författare)
- Alimentary Pharmabiotic Centre (APC), Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland
-
- Stanton, Catherine (författare)
- Alimentary Pharmabiotic Centre (APC), Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland
-
- O'Mahony, Liam (författare)
- Alimentary Pharmabiotic Centre (APC), Cork, Ireland
-
visa fler...
-
- Wall, Rebecca, 1979- (författare)
- Alimentary Pharmabiotic Centre (APC), Cork, Ireland,Nutrition-Gut-Brain Interactions Research Centre
-
- Shanahan, Fergus (författare)
- Alimentary Pharmabiotic Centre (APC), Cork, Ireland
-
- Quigley, Eamonn M. (författare)
- Alimentary Pharmabiotic Centre (APC), Cork, Ireland
-
- Fitzgerald, Gerald F. (författare)
- Alimentary Pharmabiotic Centre (APC), Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland
-
- Ross, R. Paul (författare)
- Alimentary Pharmabiotic Centre (APC), Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland
-
visa färre...
-
(creator_code:org_t)
- Berks, United Kingdom : Society for General Microbiology, 2011
- 2011
- Engelska.
-
Ingår i: Microbiology. - Berks, United Kingdom : Society for General Microbiology. - 1350-0872 .- 1465-2080. ; 157:2, s. 609-615
- Relaterad länk:
-
http://mic.microbiol...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- We have previously demonstrated that oral administration of a metabolically active Bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (CLA), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA and omega-3 (n-3) fatty acids in liver and adipose tissue. In this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production of t10, c12 CLA) from Propionibacterium acnes (PAI) could influence the fatty acid composition of different tissues in a mouse model. Linoleic-acid-supplemented diets (2 %, w/w) were fed in combination with either a recombinant t10, c12 CLA-producing Lactobacillus paracasei NFBC 338 (Lb338), or an isogenic (vector-containing) control strain, to BALB/c mice for 8 weeks. A third group of mice received linoleic acid alone (2 %, w/w). Tissue fatty acid composition was assessed by GLC at the end of the trial. Ingestion of the strain expressing linoleic acid isomerase was associated with a 4-fold increase (P<0.001) in t10, c12 CLA in adipose tissues of the mice when compared with mice that received the isogenic non-CLA-producing strain. The livers of the mice that received the recombinant CLA-producing Lb338 also contained a 2.5-fold (albeit not significantly) higher concentration of t10, c12 CLA, compared to the control group. These data demonstrate that a single gene (encoding linoleic acid isomerase) expressed in an intestinal microbe can influence the fatty acid composition of host fat.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas