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Recombinant lactoba...
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Rosberg-Cody, EvaAlimentary Pharmabiotic Centre (APC), Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland
(författare)
Recombinant lactobacilli expressing linoleic acid isomerase can modulate the fatty acid composition of host adipose tissue in mice
- Artikel/kapitelEngelska2011
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Berks, United Kingdom :Society for General Microbiology,2011
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Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:oru-46363
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-46363URI
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https://doi.org/10.1099/mic.0.043406-0DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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We have previously demonstrated that oral administration of a metabolically active Bifidobacterium breve strain, with ability to form cis-9, trans-11 conjugated linoleic acid (CLA), resulted in modulation of the fatty acid composition of the host, including significantly elevated concentrations of c9, t11 CLA and omega-3 (n-3) fatty acids in liver and adipose tissue. In this study, we investigated whether a recombinant lactobacillus expressing linoleic acid isomerase (responsible for production of t10, c12 CLA) from Propionibacterium acnes (PAI) could influence the fatty acid composition of different tissues in a mouse model. Linoleic-acid-supplemented diets (2 %, w/w) were fed in combination with either a recombinant t10, c12 CLA-producing Lactobacillus paracasei NFBC 338 (Lb338), or an isogenic (vector-containing) control strain, to BALB/c mice for 8 weeks. A third group of mice received linoleic acid alone (2 %, w/w). Tissue fatty acid composition was assessed by GLC at the end of the trial. Ingestion of the strain expressing linoleic acid isomerase was associated with a 4-fold increase (P<0.001) in t10, c12 CLA in adipose tissues of the mice when compared with mice that received the isogenic non-CLA-producing strain. The livers of the mice that received the recombinant CLA-producing Lb338 also contained a 2.5-fold (albeit not significantly) higher concentration of t10, c12 CLA, compared to the control group. These data demonstrate that a single gene (encoding linoleic acid isomerase) expressed in an intestinal microbe can influence the fatty acid composition of host fat.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Stanton, CatherineAlimentary Pharmabiotic Centre (APC), Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland
(författare)
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O'Mahony, LiamAlimentary Pharmabiotic Centre (APC), Cork, Ireland
(författare)
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Wall, Rebecca,1979-Alimentary Pharmabiotic Centre (APC), Cork, Ireland,Nutrition-Gut-Brain Interactions Research Centre(Swepub:oru)rwl
(författare)
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Shanahan, FergusAlimentary Pharmabiotic Centre (APC), Cork, Ireland
(författare)
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Quigley, Eamonn M.Alimentary Pharmabiotic Centre (APC), Cork, Ireland
(författare)
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Fitzgerald, Gerald F.Alimentary Pharmabiotic Centre (APC), Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland
(författare)
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Ross, R. PaulAlimentary Pharmabiotic Centre (APC), Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland
(författare)
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Alimentary Pharmabiotic Centre (APC), Cork, Ireland; Department of Microbiology, University College Cork, Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, IrelandAlimentary Pharmabiotic Centre (APC), Cork, Ireland; Teagasc Moorepark Food Research Centre, Fermoy, Ireland
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:MicrobiologyBerks, United Kingdom : Society for General Microbiology157:2, s. 609-6151350-08721465-2080
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