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Analytical specific...
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Golparian, Daniel,1984-WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden
(author)
Analytical specificity and sensitivity of the novel dual-target GeneProof Neisseria gonorrhoeae PCR kit for detection of N-gonorrhoeae
- Article/chapterEnglish2015
Publisher, publication year, extent ...
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2015-09-02
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Wiley-Blackwell,2015
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:oru-46695
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-46695URI
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https://doi.org/10.1111/apm.12440DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Detection of Neisseria gonorrhoeae relies increasingly on nucleic acid amplification tests (NAATs). The specificity of many gonococcal NAATs has been suboptimal and supplementary testing remains recommended in Europe and several additional countries. The novel dual-target GeneProofNeisseria gonorrhoeae PCR kit, targeting porA pseudogene and 16S rRNA gene, showed a high specificity and sensitivity when isolates of non-gonococcal Neisseria and related species (n=144), and gonococci (n=104) were tested. However, rare gonococcal porA mutants were only detected in the 16S rRNA gene target and two non-gonococcal isolates showed a low-level cross-reactivity in the 16S rRNA gene target. The detection limit for both targets was 1.5 copies per reaction.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Hellmark, Bengt,1971-Örebro universitet,Institutionen för hälsovetenskap och medicin,WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden(Swepub:oru)bhk
(author)
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Unemo, Magnus,1970-Örebro universitet,Institutionen för hälsovetenskap och medicin,WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, Sweden(Swepub:oru)muo
(author)
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WHO Collaborating Centre for Gonorrhoea and other Sexually Transmitted Infections, Swedish Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Faculty of Medicine and Health, Örebro University, Örebro, SwedenInstitutionen för hälsovetenskap och medicin
(creator_code:org_t)
Related titles
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In:Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS): Wiley-Blackwell123:11, s. 955-9580903-46411600-0463
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