Search: (AMNE:(Clinical Medicine)) >
Real-time PCR of th...
Real-time PCR of the 16S-rRNA gene in the diagnosis of neonatal bacteraemia
- Article/chapterEnglish2008
Publisher, publication year, extent ...
-
Oslo :Taylor & Francis,2008
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:oru-5149
-
https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-5149URI
-
https://doi.org/10.1111/j.1651-2227.2008.00924.xDOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
OBJECTIVE: To evaluate a real-time PCR assay for the diagnosis of neonatal bacteraemia. PATIENTS AND METHODS: Two hundred ninety-five plasma samples from 288 newborns with suspected neonatal sepsis were collected prospectively for the purpose of polymerase chain reaction (PCR)-based bacterial detection. A real-time PCR targeting the bacterial gene for 16S-rRNA gene combined with four specific probes designed to detect Gram-negative bacteria, Staphylococcus aureus and coagulase-negative staphylococci (CoNS) was developed. All samples positive in the universal PCR were further sequenced for bacterial identification. RESULTS: When applied to a material from 50 patients with positive blood culture and 245 patients with negative blood culture, the universal PCR showed a sensitivity of 42% (28-57), a specificity of 95% (92-97), a positive predictive value of 64% (45-80), and a negative predictive value of 89% (84-92) (95% confidence intervals in brackets). CONCLUSION: A new real-time PCR technique was for the first time applied to a well-defined prospectively and consecutively enrolled material of newborns with suspected sepsis, combining the benefits of real-time PCR with specific probes and sequencing. The method managed to detect bacteraemia with high specificity even though the sensitivity was low. Factors causing the low sensitivity are identified and further strategies to develop the method are described.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Bäckman, Anders
(author)
-
Björkqvist, Maria
(author)
-
Mölling, Paula
(author)
-
Jurstrand, Margaretha
(author)
-
Schollin, JensÖrebro universitet,Hälsoakademin(Swepub:oru)jssn
(author)
-
Örebro universitetHälsoakademin
(creator_code:org_t)
Related titles
-
In:Acta PaediatricaOslo : Taylor & Francis97:10, s. 1376-13800803-52531651-2227
Internet link
Find in a library
To the university's database