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Sökning: onr:"swepub:oai:DiVA.org:oru-52206" > Formulation and sta...

  • Melin, PetterSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology (författare)

Formulation and stabilisation of the biocontrol yeast Pichia anomala

  • Artikel/kapitelEngelska2011

Förlag, utgivningsår, omfång ...

  • 2010-10-28
  • Dordrecht, Netherlands :Springer Netherlands,2011
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:oru-52206
  • https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-52206URI
  • https://doi.org/10.1007/s10482-010-9522-5DOI
  • https://res.slu.se/id/publ/39115URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Funding Agency:Foundation for Strategic Environmental Research-MISTRA, Sweden 
  • The yeast Pichia anomala has antifungal activities and its potential in biocontrol and biopreservation has previously been demonstrated. To practically use an organism in such applications on a larger scale the microbe has to be formulated and stabilised. In this review we give an overview of our experience of formulating and stabilising P. anomala strain J121 in a wider perspective. The stabilisation techniques we have evaluated were liquid formulations, fluidised bed drying, lyophilisation (freeze-drying) and vacuum drying. With all methods tested it was possible to obtain yeast cells with shelf lives of at least a few months and in all cases the biocontrol activity was retained. Fluidised bed drying was dependent on the addition of cottonseed flour as a carrier during the drying process. In liquid formulations a sugar, preferentially trehalose, was a required additive. These two kinds of microbial stabilisation are easily performed and relatively inexpensive but in order to keep the cells viable the biomaterial has to be stored at cool temperatures. However, there is room for optimization, such as improving the growth conditions, or include preconditioning steps to enable the cells to produce more compatible solutes necessary to survive formulation, desiccation and storage. In contrast, lyophilisation and vacuum drying require a lot of energy and are thus expensive. On the other hand, the dried cells were mostly intact after one year of storage at 30A degrees C. Inevitably, the choice of formulation and stabilisation techniques will be dependent also on the intended use.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Schnürer, Johan,1957-Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology(Swepub:slu)46771 (författare)
  • Håkansson, SebastianSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology(Swepub:slu)48672 (författare)
  • Sveriges lantbruksuniversitetInstitutionen för mikrobiologi (creator_code:org_t)
  • Sveriges lantbruksuniversitet

Sammanhörande titlar

  • Ingår i:Antonie van Leeuwenhoek. International Journal of General and Molecular MicrobiologyDordrecht, Netherlands : Springer Netherlands99:1, s. 107-1120003-60721572-9699

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