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Performance of myco...
Performance of mycological media in enumerating desiccated food spoilage yeasts : an interlaboratory study
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- Beuchat, L. R. (författare)
- Center for Food Safety and Department of Food Science and Technology, University of Georgia, Griffin, GA, USA
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- Frändberg, E. (författare)
- Biology Division, National Food Administration, Uppsala, Sweden
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- Deak, T. (författare)
- Center for Food Safety and Department of Food Science and Technology, University of Georgia, Griffin, GA, USA; Department of Microbiology and Biotechnology, Szent Istvan University, Budapest, Hungary
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- Alzamora, S. M. (författare)
- Pabellon de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
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- Chen, J. (författare)
- Center for Food Safety and Department of Food Science and Technology, University of Georgia, Griffin, GA, USA
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- Guerrero, S. (författare)
- Pabellon de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
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- López-Malo, A. (författare)
- Departmento de Ingenieria Quimica y Alimentos, Universidad de la Americas-Puebla, Sta. Catarina Martir, Puebla, Mexico
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- Ohlsson, I. (författare)
- Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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- Olsen, M. (författare)
- Biology Division, National Food Administration, Uppsala, Sweden
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- Peinado, J. M. (författare)
- Departmento de Microbiologia, Facultad de Biologia, Universidad Completense de Madrid, Madrid, Spain
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- Schnürer, Johan, 1957- (författare)
- Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
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- de Siloniz, M. I. (författare)
- Departmento de Microbiologia, Facultad de Biologia, Universidad Completense de Madrid, Madrid, Spain
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- Tornai-Lehoczki, J. (författare)
- National Collection of Agricultural and Industrial Microorganisms, Szent Istvan University, Budapest, Hungary
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(creator_code:org_t)
- Elsevier, 2001
- 2001
- Engelska.
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Ingår i: International Journal of Food Microbiology. - : Elsevier. - 0168-1605 .- 1879-3460. ; 70:1-2, s. 89-96
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Dichloran 18% glycerol agar (DG18) was originally formulated to enumerate nonfastidious xerophilic moulds in foods containing rapidly growing Eurotium species. Some laboratories are now using DG18 as a general purpose medium for enumerating yeasts and moulds, although its performance in recovering yeasts from dry foods has not been evaluated. An interlaboratory study compared DG18 with dichloran rose bengal chloramphenicol agar (DRBC), plate count agar supplemented with chloramphenicol (PCAC), tryptone glucose yeast extract chloramphenicol agar (TGYC), acidified potato dextrose agar (APDA), and orange serum agar (OSA) for their suitability to enumerate 14 species of lyophilized yeasts. The coefficient of variation for among-laboratories repeatability within yeast was 1.39% and reproducibility of counts among laboratories was 7.1%. The order of performance of media for recovering yeasts was TGYC > PCAC = OSA > APDA > DRBC > DG18. A second study was done to determine the combined effects of storage time and temperature on viability of yeasts and suitability of media for recovery. Higher viability was retained at - 18 degreesC than at 5 degreesC or 25 degreesC for up to 42 weeks, although the difference in mean counts of yeasts stored at - 18 degreesC and 25 degreesC was only 0.78 log(10) cfu/ml of rehydrated suspension. TGYC was equal to PCAC and superior to the other four media in recovering yeasts stored at - 18 degreesC, 5 degreesC, or 25 degreesC for up to 42 weeks. Results from both the interlaboratory study and the storage study support the use of TGYC for enumerating desiccated yeasts. DG18 is not recommended as a general purpose medium for recovering yeasts from a desiccated condition.
Ämnesord
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Livsmedelsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Food Science (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- mycological media
- yeast
- TGYC
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- Av författaren/redakt...
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Beuchat, L. R.
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Frändberg, E.
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Deak, T.
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Alzamora, S. M.
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Chen, J.
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Guerrero, S.
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López-Malo, A.
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Ohlsson, I.
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Olsen, M.
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Peinado, J. M.
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Schnürer, Johan, ...
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de Siloniz, M. I ...
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Tornai-Lehoczki, ...
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