Genetic characterization of the nonconventional yeast Hansenula anomala

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Search: WFRF:(Elena S) > (2000-2004) > Genetic characteriz...

Details
MARC

Naumov, Gennadi I.State Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia; Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden (author)

Genetic characterization of the nonconventional yeast Hansenula anomala

Article/chapterEnglish2001

Publisher, publication year, extent ...

Elsevier,2001
printrdacarrier

Numbers

LIBRIS-ID:oai:DiVA.org:oru-52306
https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-52306URI
https://doi.org/10.1016/S0923-2508(01)01229-3DOI

Supplementary language notes

Language:English
Summary in:English

Part of subdatabase

SwePubSwePub

Classification

Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

We describe genetic, molecular and taxonomic characteristics of the yeast Hansenula anomala. Pulsed-field gel electrophoresis of chromosomal DNAs from 19 H. anomala strains and related species indicated that H. anomala had a clearly different karyotype. Chromosome length polymorphism of the H. anomala strains was independent of their geographic origin and source of isolation. The strains were classified into four groups of similar karyotypes and one strain showed a unique profile. The sizes of chromosomes ranged from 850 to 3500 kb in different strains. The haploid chromosome number of H. anomala is at least nine. We have found RAPD primers discriminating at both the species and strain levels. All the primers tested except the M13 core sequence generated unique patterns with most strains. The results indicate the usefulness of PCR analysis with primer M13 for identification of the H. anomala species. Screening of the CBS (Utrecht) collection strains of H. anomala showed that they are rather difficult objects for genetic hybridization analysis. The strains have low fertility, viz. very poor sporulation, low mating type activities and, as a rule, nonviable ascospores. The majority of the hybrids obtained are polyploid, probably tetraploid, as judged by the segregation of control auxotrophic markers. Nevertheless, some monosporic cultures of the strains studied, including the biocontrol yeast J121, formed diploid hybrids with regular meiotic segregation of control auxotrophic markers. As a rule, H. anomala isolates are homothallic, showing delayed self-diploidization. Rare stable heterothallic strains of H. anomala also occur.

Subject headings and genre

NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
Hansenula anomala
Pichia anomala
taxogenetics
polyploidy
karyotyping
PCR analysis
D1-D2 sequence

Added entries (persons, corporate bodies, meetings, titles ...)

Naumova, Elena S.State Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia; Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden (author)
Schnürer, Johan,1957-Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden(Swepub:oru)jhsr (author)
State Institute for Genetics and Selection of Industrial Microorganisms, Moscow, Russia; Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, SwedenDepartment of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden (creator_code:org_t)

Related titles

In:Research in Microbiology: Elsevier152:6, s. 551-5620923-25081769-7123

Internet link

Find in a library

Research in Microbiology (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Naumov, Gennadi ...; Naumova, Elena S ...; Schnürer, Johan, ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

Articles in the publication: Research in Micr ...

By the university: Örebro University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se