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- Abdeldaim, Guma M. K.Uppsala universitet,Klinisk bakteriologi (author)
Quantitative fucK gene polymerase chain reaction on sputum and nasopharyngeal secretions to detect Haemophilus influenzae pneumonia
- Article/chapterEnglish2013
Publisher, publication year, extent ...
- Elsevier,2013
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:oru-56356
- https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-56356URI
- https://doi.org/10.1016/j.diagmicrobio.2013.02.015DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:126880733URI
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-204287URI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
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Notes
- Funding Agency:Regional Research Council Uppsala-Örebro
- A quantitative polymerase chain reaction (PCR) for the fucK gene was developed for specific detection of Haemophilus influenzae. The method was tested on sputum and nasopharyngeal aspirate (NPA) from 78 patients with community-acquired pneumonia (CAP). With a reference standard of sputum culture and/or serology against the patient's own nasopharyngeal isolate, H. influenzae etiology was detected in 20 patients. Compared with the reference standard, fucK PCR (using the detection limit 10(5) DNA copies/mL) on sputum and NPA showed a sensitivity of 95.0% (19/20) in both cases, and specificities of 87.9% (51/58) and 89.5% (52/58), respectively. In a receiver operating characteristic curve analysis, sputum fucK PCR was found to be significantly superior to sputum P6 PCR for detection of H. influenzae CAP. NPA fucK PCR was positive in 3 of 54 adult controls without respiratory symptoms. In conclusion, quantitative fucK real-time PCR provides a sensitive and specific identification of H. influenzae in respiratory secretions.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Infektionsmedicin hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Infectious Medicine hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
- H. influenzae
- Pneumonia
- Real-time PCR
- fucK
Added entries (persons, corporate bodies, meetings, titles ...)
- Strålin, KristofferKarolinska Institutet (author)
- Olcén, PerDepartment of Laboratory Medicine, Örebro University Hospital, Örebro, Sweden (author)
- Blomberg, JonasUppsala universitet,Klinisk virologi(Swepub:uu)jonablom (author)
- Mölling, PaulaRegion Örebro län,Department of Laboratory Medicine(Swepub:oru)OUH-pa1mo (author)
- Herrmann, BjörnUppsala universitet,Klinisk bakteriologi(Swepub:uu)bjorherr (author)
- Uppsala universitetKlinisk bakteriologi (creator_code:org_t)
Related titles
- In:Diagnostic microbiology and infectious disease: Elsevier76:2, s. 141-1460732-88931879-0070
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