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  • Seth-Smith, Helena M. B.Wellcome Trust Sanger Inst, Hinxton, England (författare)

Whole-genome sequences of Chlamydia trachomatis directly from clinical samples without culture

  • Artikel/kapitelEngelska2013

Förlag, utgivningsår, omfång ...

  • 2013-03-22
  • Cold Spring Harbor Laboratory Press (CSHL),2013
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:oru-56736
  • https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-56736URI
  • https://doi.org/10.1101/gr.150037.112DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • The use of whole-genome sequencing as a tool for the study of infectious bacteria is of growing clinical interest. Chlamydia trachomatis is responsible for sexually transmitted infections and the blinding disease trachoma, which affect hundreds of millions of people worldwide. Recombination is widespread within the genome of C. trachomatis, thus whole-genome sequencing is necessary to understand the evolution, diversity, and epidemiology of this pathogen. Culture of C trachomatis has, until now, been a prerequisite to obtain DNA for whole-genome sequencing; however, as C trachomatis is an obligate intracellular pathogen, this procedure is technically demanding and time consuming. Discarded clinical samples represent a large resource for sequencing the genomes of pathogens, yet clinical swabs frequently contain very low levels of C trachomatis DNA and large amounts of contaminating microbial and human DNA. To determine whether it is possible to obtain whole-genome sequences from bacteria without the need for culture, we have devised an approach that combines immunomagnetic separation (IMS) for targeted bacterial enrichment with multiple displacement amplification (MDA) for whole-genome amplification. Using IMS-MDA ill conjunction with high-throughput multiplexed Illumina sequencing, we have produced the first whole bacterial genome sequences direct from clinical samples. We also show that this method can be used to generate genome data from nonviable archived samples. This method will prove a useful tool in answering questions relating to the biology of many difficult-to-culture or fastidious bacteria of clinical concern.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Harris, Simon R.Wellcome Trust Sanger Inst, Hinxton, England (författare)
  • Skilton, Rachel J.Fac Med, Mol Microbiol Grp, Southampton Gen Hosp, Univ Southampton, Southampton, England (författare)
  • Radebe, Frans M.Natl Inst Communicable Dis, Ctr HIV & Sexually Transmitted Infect, National Health Laboratory Service, Johannesburg, South Africa (författare)
  • Golparian, Daniel,1984-Natl Reference Lab Pathogen Neisseria, WHO Collaborating Ctr Gonorrhoea & Other STIs, Örebro University Hospital, Örebro, Sweden(Swepub:oru)degn (författare)
  • Shipitsyna, ElenaLab Microbiol, DO Ott Res Inst Obstet & Gynaecol, St Petersburg, Russia (författare)
  • Duy, Pham Thanh (författare)
  • Scott, PaulWellcome Trust Sanger Inst, Hinxton, England (författare)
  • Cutcliffe, Lesley T.Fac Med, Mol Microbiol Grp, Southampton Gen Hosp, Univ Southampton, Southampton, England (författare)
  • O'Neill, ColetteFac Med, Mol Microbiol Grp, Southampton Gen Hosp, Univ Southampton, Southampton, England (författare)
  • Parmar, SurendraHlth Protect Agcy, Clin Microbiol & Publ Hlth Lab, Addenbrookes Hosp, Cambridge, England (författare)
  • Pitt, RachelSexually Transmitted Bacteria Reference Lab, Health Protection Agency, London, England (författare)
  • Baker, StephenClin Res Unit, Wellcome Trust Major Overseas List, Hosp Trop Dis, Univ Oxford, Ho Chi Minh City, Vietnam (författare)
  • Ison, Catherine A.Sexually Transmitted Bacteria Reference Lab, Health Protection AgencyLondon NW9 5HT, England. (författare)
  • Marsh, PeterPubl Hlth Lab Southampton, Health Protection Agency, Southampton Gen Hosp, Southampton, England (författare)
  • Jalal, HamidHealth Protection Agency, Clin Microbiol & Publ Hlth Lab, Addenbrookes Hosp, Cambridge, England (författare)
  • Lewis, David A.Natl Inst Communicable Dis, Ctr HIV & Sexually Transmitted Infect, National Health Laboratory ServiceJohannesburg, South Africa; Fac Hlth Sci, Dept Internal Med, Univ Witwatersrand, Johannesburg, South Africa (författare)
  • Unemo, MagnusRegion Örebro län,Natl Reference Lab Pathogen Neisseria, WHO Collaborating Ctr Gonorrhoea & Other STIs, Örebro University Hospital, Örebro, Sweden(Swepub:oru)muo (författare)
  • Clarke, Ian N.Fac Med, Mol Microbiol Grp, Southampton Gen Hosp, Univ Southampton, Southampton, England (författare)
  • Parkhill, JulianWellcome Trust Sanger Inst, Hinxton, England (författare)
  • Thomson, Nicholas R.Wellcome Trust Sanger Inst, Hinxton, England (författare)
  • Wellcome Trust Sanger Inst, Hinxton, EnglandFac Med, Mol Microbiol Grp, Southampton Gen Hosp, Univ Southampton, Southampton, England (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Genome Research: Cold Spring Harbor Laboratory Press (CSHL)23:5, s. 855-8661088-90511549-5469

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