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Remodeling of centr...
Remodeling of central metabolism in invasive breast cancer compared to normal breast tissue - a GC-TOFMS based metabolomics study
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- Budczies, Jan (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
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- Denkert, Carsten (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
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- Müller, Berit M. (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
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- Brockmöller, Scarlet F. (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
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- Klauschen, Frederick (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
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- Györffy, Balazs (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany; Institute of Pathology, Charité University Hospital, Berlin, Germany
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- Dietel, Manfred (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
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- Richter-Ehrenstein, Christiane (författare)
- Interdisciplinary Breast Center, Charité University Hospital, Berlin, Germany
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- Marten, Ulrike (författare)
- Institute of Pathology, DRK Kliniken Berlin, Berlin, Germany
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- Salek, Reza M. (författare)
- Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
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- Griffin, Julian L. (författare)
- Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
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- Hilvo, Mika (författare)
- VTT Technical Research Centre of Finland, Espoo, Finland
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- Oresic, Matej, 1967- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,VTT Technical Research Centre of Finland, Espoo, Finland
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- Wohlgemuth, Gert (författare)
- Genome Center, University of California Davis, Davis CA, United States
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- Fiehn, Oliver (författare)
- Genome Center, University of California Davis, Davis CA, United States
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(creator_code:org_t)
- Springer Science and Business Media LLC, 2012
- 2012
- Engelska.
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Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 13:1
- Relaterad länk:
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https://doi.org/10.1...
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https://bmcgenomics....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- BACKGROUND: Changes in energy metabolism of the cells are common to many kinds of tumors and are considered a hallmark of cancer. Gas chromatography followed by time-of-flight mass spectrometry (GC-TOFMS) is a well-suited technique to investigate the small molecules in the central metabolic pathways. However, the metabolic changes between invasive carcinoma and normal breast tissues were not investigated in a large cohort of breast cancer samples so far.RESULTS: A cohort of 271 breast cancer and 98 normal tissue samples was investigated using GC-TOFMS-based metabolomics. A total number of 468 metabolite peaks could be detected; out of these 368 (79%) were significantly changed between cancer and normal tissues (p<0.05 in training and validation set). Furthermore, 13 tumor and 7 normal tissue markers were identified that separated cancer from normal tissues with a sensitivity and a specificity of >80%. Two-metabolite classifiers, constructed as ratios of the tumor and normal tissues markers, separated cancer from normal tissues with high sensitivity and specificity. Specifically, the cytidine-5-monophosphate / pentadecanoic acid metabolic ratio was the most significant discriminator between cancer and normal tissues and allowed detection of cancer with a sensitivity of 94.8% and a specificity of 93.9%.CONCLUSIONS: For the first time, a comprehensive metabolic map of breast cancer was constructed by GC-TOF analysis of a large cohort of breast cancer and normal tissues. Furthermore, our results demonstrate that spectrometry-based approaches have the potential to contribute to the analysis of biopsies or clinical tissue samples complementary to histopathology.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
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- Av författaren/redakt...
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Budczies, Jan
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Denkert, Carsten
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Müller, Berit M.
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Brockmöller, Sca ...
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Klauschen, Frede ...
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Györffy, Balazs
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visa fler...
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Dietel, Manfred
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Richter-Ehrenste ...
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Marten, Ulrike
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Salek, Reza M.
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Griffin, Julian ...
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Hilvo, Mika
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Oresic, Matej, 1 ...
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Wohlgemuth, Gert
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Fiehn, Oliver
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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BMC Genomics
- Av lärosätet
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Örebro universitet