Sökning: WFRF:(Richter Ulrike) >
Remodeling of centr...
Remodeling of central metabolism in invasive breast cancer compared to normal breast tissue - a GC-TOFMS based metabolomics study
-
- Budczies, Jan (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
-
- Denkert, Carsten (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
-
- Müller, Berit M. (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
-
visa fler...
-
- Brockmöller, Scarlet F. (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
-
- Klauschen, Frederick (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
-
- Györffy, Balazs (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany; Institute of Pathology, Charité University Hospital, Berlin, Germany
-
- Dietel, Manfred (författare)
- Institute of Pathology, Charité University Hospital, Berlin, Germany
-
- Richter-Ehrenstein, Christiane (författare)
- Interdisciplinary Breast Center, Charité University Hospital, Berlin, Germany
-
- Marten, Ulrike (författare)
- Institute of Pathology, DRK Kliniken Berlin, Berlin, Germany
-
- Salek, Reza M. (författare)
- Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
-
- Griffin, Julian L. (författare)
- Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom
-
- Hilvo, Mika (författare)
- VTT Technical Research Centre of Finland, Espoo, Finland
-
- Oresic, Matej, 1967- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper,VTT Technical Research Centre of Finland, Espoo, Finland
-
- Wohlgemuth, Gert (författare)
- Genome Center, University of California Davis, Davis CA, United States
-
- Fiehn, Oliver (författare)
- Genome Center, University of California Davis, Davis CA, United States
-
visa färre...
-
(creator_code:org_t)
- Springer Science and Business Media LLC, 2012
- 2012
- Engelska.
-
Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 13:1
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://bmcgenomics....
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- BACKGROUND: Changes in energy metabolism of the cells are common to many kinds of tumors and are considered a hallmark of cancer. Gas chromatography followed by time-of-flight mass spectrometry (GC-TOFMS) is a well-suited technique to investigate the small molecules in the central metabolic pathways. However, the metabolic changes between invasive carcinoma and normal breast tissues were not investigated in a large cohort of breast cancer samples so far.RESULTS: A cohort of 271 breast cancer and 98 normal tissue samples was investigated using GC-TOFMS-based metabolomics. A total number of 468 metabolite peaks could be detected; out of these 368 (79%) were significantly changed between cancer and normal tissues (p<0.05 in training and validation set). Furthermore, 13 tumor and 7 normal tissue markers were identified that separated cancer from normal tissues with a sensitivity and a specificity of >80%. Two-metabolite classifiers, constructed as ratios of the tumor and normal tissues markers, separated cancer from normal tissues with high sensitivity and specificity. Specifically, the cytidine-5-monophosphate / pentadecanoic acid metabolic ratio was the most significant discriminator between cancer and normal tissues and allowed detection of cancer with a sensitivity of 94.8% and a specificity of 93.9%.CONCLUSIONS: For the first time, a comprehensive metabolic map of breast cancer was constructed by GC-TOF analysis of a large cohort of breast cancer and normal tissues. Furthermore, our results demonstrate that spectrometry-based approaches have the potential to contribute to the analysis of biopsies or clinical tissue samples complementary to histopathology.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Budczies, Jan
-
Denkert, Carsten
-
Müller, Berit M.
-
Brockmöller, Sca ...
-
Klauschen, Frede ...
-
Györffy, Balazs
-
visa fler...
-
Dietel, Manfred
-
Richter-Ehrenste ...
-
Marten, Ulrike
-
Salek, Reza M.
-
Griffin, Julian ...
-
Hilvo, Mika
-
Oresic, Matej, 1 ...
-
Wohlgemuth, Gert
-
Fiehn, Oliver
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinsk biotek ...
-
och Medicinsk biotek ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Cancer och onkol ...
- Artiklar i publikationen
-
BMC Genomics
- Av lärosätet
-
Örebro universitet