Response of protein synthesis in human skeletal muscle to insulin: an investigation with L[2H5]phenylalanine

McNurlan, Margaret A.Rowett Research Institute, Aberdeen, United Kingdom. (författare)

Response of protein synthesis in human skeletal muscle to insulin : an investigation with L[2H5]phenylalanine

The following affiliations are found in the publication without connection to their respective person:Rowlett Research Institute, Aberdeen,ScotlandKarolinska Hosp,Dept Surg, Stockholm,SwedenHuddinge Hosp,Dept Anesthesiol,Huddinge,SwedenSt Gorans Univ Hosp,Dept Anesthesiol, Stockholm,SwedenUniv Bern,Inselspital,Dept Internal Med, Bern,Switzerland
The role of insulin in the regulation of muscle protein synthesis in adult humans has been investigated with intravenous infusion of insulin at levels comparable with those observed after normal feeding. Glucose was also infused to maintain euglycemia. Muscle protein synthesis was measured in six healthy subjects before and during insulin and glucose infusion from the incorporation of L-[H-2(5)]phenylalanine into the protein of vastus lateralis sampled by percutaneous biopsy. L-[H-2(5)]phenylalanine was given as a single injection of a flooding amount (45 mg/kg). The relatively low levels of enrichment of phenylalanine in protein (0.005 atom%) were measured by modified gas chromatography-mass spectrometry and verified by comparison with incorporation of L-[2,6-H-3]phenylalanine. Similarity of enrichment in tissue-free and plasma pools (flooding) and linear incorporation over the period of measurement were also verified. The fractional rate of muscle protein synthesis in the group of postabsorptive subjects was 1.65 +/- 0.11% (SE)/day. The rate was unaltered by insulin and glucose infusion, 1.66 +/- 0.16%/day.

Essén, Pia (författare)
Thorell, Anders (författare)
Calder, A. Graham (författare)
Andersson, S. E. (författare)
Ljungqvist, Olle,1954-Örebro universitet,Institutionen för medicinska vetenskaper(Swepub:oru)olt (författare)
Sandgren, Anna (författare)
Grant, Ian (författare)
Tjäder, Inga (författare)
Ballmer, Peter E. (författare)
Wernerman, Jan (författare)
Garlick, Peter J. (författare)
Rowett Research Institute, Aberdeen, United Kingdom.Institutionen för medicinska vetenskaper (creator_code:org_t)

Ingår i:American Journal of Physiology: American Physiological Society67:Part 1, s. E102-E1080002-95132163-5773

Av författaren/redakt...: McNurlan, Margar ...; Essén, Pia; Thorell, Anders; Calder, A. Graha ...; Andersson, S. E.; Ljungqvist, Olle ...; visa fler...; Sandgren, Anna; Grant, Ian; Tjäder, Inga; Ballmer, Peter E ...; Wernerman, Jan; Garlick, Peter J ...; visa färre...

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