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Platelet adhesion c...
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Tynngård, NahreenLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten,Region Östergötland, Klinisk immunologi och transfusionsmedicin,Region Östergötland, Klinisk kemi
(författare)
Platelet adhesion changes during storage studied with a novel method using flow cytometry and protein-coated beads
- Artikel/kapitelEngelska2015
Förlag, utgivningsår, omfång ...
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2014-03-28
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Taylor & Francis,2015
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printrdacarrier
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LIBRIS-ID:oai:DiVA.org:oru-66031
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-66031URI
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https://doi.org/10.3109/09537104.2014.891728DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-111533URI
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Språk:engelska
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Sammanfattning på:engelska
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The aim of the present study was to set up and evaluate a novel method for studies of platelet adhesion and activation in blood and platelet suspensions such as platelet concentrate (PC) samples using protein-coated polystyrene beads and flow cytometry. To demonstrate its usefulness, we studied PCs during storage. PCs were prepared by aphaeresis technique (n = 7). Metabolic variables and platelet function was measured on day 1, 5, 7 and 12 of storage. Spontaneous and TRAP-6-induced adhesion to fibrinogen-and collagen-coated beads was analyzed by flow cytometry. P-selectin and phosphatidyl serine (PS) expression was assessed on platelets bound to beads as well as on non-adherent platelets. Platelet adhesion to fibrinogen beads had increased by day 12 and adhesion to collagen beads at day 7 of storage (p<0.05). TRAP-6 stimulation significantly increased the platelet adhesion to fibrinogen beads (p<0.05) as well as the P-selectin and PS exposure on platelets bound to beads (p<0.01) during the first 7 days of storage, but by day 12, significant changes were no longer induced by TRAP-6 stimulation. We demonstrate that our adhesion assay using protein-coated polystyrene beads can be used to assess the adhesion properties of platelets during storage without the addition of red blood cells. Therefore it may offer a useful tool for future studies of platelet adhesive capacity in transfusion medicine and other settings.
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Wallstedt, MariaLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)marwa83
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Södergren, Anna LLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)annso15
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Faxälv, LarsLinköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten,Region Östergötland, Klinisk kemi(Swepub:liu)larfa71
(författare)
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Ramström, Sofia,1973-Linköpings universitet,Avdelningen för mikrobiologi och molekylär medicin,Medicinska fakulteten(Swepub:liu)sofra86
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Linköpings universitetAvdelningen för mikrobiologi och molekylär medicin
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Sammanhörande titlar
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Ingår i:Platelets: Taylor & Francis26:2, s. 177-1850953-71041369-1635
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