id:"swepub:oai:DiVA.org:oru-66163"
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- Ramström, Sofia,1973-Biomedical Diagnostics Institute (BDI) Programme, Molecular & Cellular Therapeutics, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland (författare)
Annexin V binding to platelets is agonist, time and temperature dependent
- Artikel/kapitelEngelska2010
Förlag, utgivningsår, omfång ...
- 2010-03-15
- Taylor & Francis,2010
- printrdacarrier
Nummerbeteckningar
- LIBRIS-ID:oai:DiVA.org:oru-66163
- https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-66163URI
- https://doi.org/10.3109/09537101003660564DOI
- https://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-71575URI
Kompletterande språkuppgifter
- Språk:engelska
- Sammanfattning på:engelska
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- SwePubSwePub
Klassifikation
Anmärkningar
- Platelets bind annexin V when stimulated with combinations of platelet agonists such as collagen and thrombin. Previous studies have demonstrated significant heterogeneity of platelets binding annexin V. The relative role of the thrombin protease-activated receptors (PARs), PAR1 and PAR4, together with different methods of platelet preparation on annexin V binding to platelets is unclear. We therefore investigated the role of PAR1- and PAR4-activating peptides in combination with collagen-related peptide on annexin V binding. In diluted whole blood, PAR1- and PAR4-activating peptides were as effective as thrombin in inducing annexin V binding. However, in washed platelets, PAR-activating peptides were less potent than thrombin at inducing annexin V binding. This difference was more pronounced when experiments were performed at 37 degrees C compared to room temperature. In studies of diluted whole blood, platelet rich plasma and washed platelets, platelets incubated at room temperature bound more annexin V than platelets incubated at 37 degrees C. We also saw a significant effect of time on annexin V binding, in that more annexin V bound to platelets with longer incubation times. In conclusion, PAR1 and PAR4-activating peptides were as effective as thrombin in inducing annexin V binding in combination with collagen-related peptide in diluted whole blood and platelet rich plasma, but not in washed platelets. In addition, incubation temperature and time has a strong influence on annexin V binding to platelets. Thus variations in these conditions may explain the differences observed between previous studies.
Ämnesord och genrebeteckningar
- NATURVETENSKAP Biologi Cellbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Cell Biology hsv//eng
- Blood platelets
- annexin V
- flow cytometry
- PAR1
- PAR4
- GPVI
Biuppslag (personer, institutioner, konferenser, titlar ...)
- O'Neill, SarahBiomedical Diagnostics Institute (BDI) Programme, Molecular & Cellular Therapeutics, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland (författare)
- Dunne, EimearBiomedical Diagnostics Institute (BDI) Programme, Molecular & Cellular Therapeutics, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland (författare)
- Kenny, DermotBiomedical Diagnostics Institute (BDI) Programme, Molecular & Cellular Therapeutics, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland (författare)
- Biomedical Diagnostics Institute (BDI) Programme, Molecular & Cellular Therapeutics, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland (creator_code:org_t)
Sammanhörande titlar
- Ingår i:Platelets: Taylor & Francis21:4, s. 289-2960953-71041369-1635
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