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Twin studies reveal...
Twin studies reveal specific imbalances in the mucosa-associated microbiota of patients with ileal Crohn's disease
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Willing, Ben (author)
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- Halfvarson, Jonas (author)
- Örebro universitet,Hälsoakademin
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- Dicksved, Johan (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology
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- Rosenquist, Magnus (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Uppsala universitet,Enheten för klinisk immunologi,Institutionen för mikrobiologi,Department of Microbiology
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- Järnerot, Gunnar (author)
- Örebro universitet,Hälsoakademin
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- Engstrand, Lars (author)
- Karolinska Institutet
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- Tysk, Curt (author)
- Örebro universitet,Hälsoakademin
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- Jansson, Janet (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för mikrobiologi,Department of Microbiology
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(creator_code:org_t)
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- New York : John Wiley & Sons, 2009
- 2009
- English.
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In: Inflammatory Bowel Diseases. - New York : John Wiley & Sons. - 1078-0998 .- 1536-4844. ; 15:5, s. 653-660
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Abstract
Subject headings
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- BACKGROUND: Large interindividual variation in the composition of the intestinal microbiota between unrelated individuals has made it challenging to identify specific aspects of dysbiosis that lead to Crohn's disease (CD).METHODS: To reduce variations in exposure during establishment of the gut flora and the influence of genotype, we studied the mucosa-associated microbiota of monozygotic twin pairs that were discordant (n = 6) or concordant (n = 4) for CD. DNA was extracted from biopsies collected from 5 locations between the ileum and rectum. Bacterial 16S ribosomal RNA genes were amplified and community composition assessed by terminal-restriction fragment length polymorphism, cloning and sequencing, and quantitative real-time polymerase chain reaction (PCR).RESULTS: The microbial compositions at all biopsy locations for each individual were similar, regardless of disease state, but there were differences between individuals. In particular, individuals with predominantly ileal CD had a dramatically lower abundance (P < 0.001) of Faecalibacterium prausnitzii and increased abundance (P < 0.03) of Escherichia coli compared to healthy co-twins and those with CD localized in the colon. This dysbiosis was significantly correlated to the disease phenotype rather than genotype.CONCLUSIONS: The reduced abundance of F. prausnitzii and increased abundance of E. coli are indicative of an ileal CD phenotype, distinct from colonic CD, and the relative abundances of these specific bacterial populations are promising biomarker candidates for differential diagnosis of CD and eventually customized treatment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Gastroenterologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Gastroenterology and Hepatology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Keyword
- MEDICINE
- MEDICIN
- Gastroenterology
- Gastroenterologi
- Internal medicine
- Invärtesmedicin
- Medicin
- Medicine
- Escherichia coli
Publication and Content Type
- ref (subject category)
- art (subject category)
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