Differential clustering of faecal and mucosa-associated microbiota in healthy individuals

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Carstens, Adam,1975-Karolinska Institutet,Örebro universitet,Institutionen för medicinska vetenskaper,Department of Gastroenterology, Faculty of Medicine and Health, Örebro University, Örebro, Sweden; Department of Internal Medicine, Ersta hospital, Stockholm, Sweden (author)

Differential clustering of faecal and mucosa-associated microbiota in healthy individuals

Article/chapterEnglish2018

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2019-01-13
Wiley-Blackwell Publishing Asia,2018
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LIBRIS-ID:oai:DiVA.org:oru-70345
https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-70345URI
https://doi.org/10.1111/1751-2980.12688DOI
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-165716URI
http://kipublications.ki.se/Default.aspx?queryparsed=id:139999746URI

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Language:English
Summary in:English

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Subject category:art swepub-publicationtype

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Funding Agencies:Örebro University Bengt Ihre Foundation Nanna Svartz Foundation Örebro University Hospital Research Foundation Örebro County Research Foundation Söderberg Foundation Swedish Foundation for Gastrointestinal Research
BACKGROUND: Faecal samples are often used to characterise gut microbiota, since they are easily collected. However, whether or not the faecal microbiota differ from the mucosa-associated microbiota remains largely unknown. This may be specifically relevant in conditions that are characterised by complex mucosal microbe-host interactions, such as Crohn's disease. We aimed to determine the degree of agreement between faecal and mucosal microbiota profiles in healthy individuals, using two commonly used collection procedures.MATERIAL AND METHODS: The gut microbiota composition of faecal samples (sent at ambient temperature before storage at -70°C) and of colonic biopsies (obtained at endoscopy and immediately stored at -70°C) was determined by sequencing the 16S rRNA gene. Thirty-one randomly selected healthy individuals from the population-based colonoscopy (Popcol) study were included.RESULTS: Faecal samples were characterised by a reduced degree of richness (p<0.0001) and diversity (p=0.016), and also differences in several phyla, including a lower relative abundance of Proteobacteria (p<0.0001) and Verrucomicrobia (p=0.008) than in biopsies. Only 3 of 30 individuals had a similar faecal and mucosal microbiota profile, based on weighted UniFrac analysis. A difference in Crohn's disease dysbiosis-associated bacteria was observed, including a lower relative abundance of Faecalibacterium (p=0.004) and a higher relative abundance of Ruminococcus (p=0.001) in faeces than in biopsies.CONCLUSIONS: Analysis of faecal samples that have been transported at ambient temperature does not adequately reflect the colonic mucosa-associated microbiota in healthy individuals. These findings have implications for the interpretation of the previous literature, and may be specifically relevant to studies on Crohn's disease.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Gastroenterologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Gastroenterology and Hepatology hsv//eng
Crohn disease
fecal microbiota
mucosa-associated microbiota

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Roos, AnnikaDepartment of Microbiology, Tumor and Cell biology & Science for Life Laboratory, Karolinska Institute, Solna, Sweden (author)
Andreasson, AnnaKarolinska Institutet,Stockholms universitet,Stressforskningsinstitutet,Science for Life Laboratory (SciLifeLab),Karolinska Institute, Sweden(Swepub:su)anan6088 (author)
Magnuson, AndersClinical Epidemiology and Biostatistics, Faculty of Medicine and Health, Örebro University, Örebro, Sweden (author)
Agréus, LarsKarolinska Institutet (author)
Halfvarson, Jonas,1970-Örebro universitet,Institutionen för medicinska vetenskaper,Department of Gastroenterology(Swepub:oru)jshn (author)
Engstrand, LarsKarolinska Institutet (author)
Örebro universitetInstitutionen för medicinska vetenskaper (creator_code:org_t)

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In:Journal of Digestive Diseases: Wiley-Blackwell Publishing Asia19:12, s. 745-7521751-29721751-2980

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